%0 Journal Article %A lashkarizadeh, Dr Mohammad Reza %A Bazrafshan, Mohammad Reza %A Izadi, Ali %A HOSEINI, FATEMEH %T Evaluation of HER2/neu gene amplification frequency in patients with gastric cancer using MLPA method %J Journal title %V 2 %N 4 %U http://jms.thums.ac.ir/article-1-264-en.html %R %D 2015 %K amplification, gene, HER2/neu, gastric cancer, MLPA method, %X Background and aims: Gastric malignancies have the fourth place among the most prevalent cancers. In many cancers, overexpressing of HER2/neu gene has been observed with a poor prediction. Up to now, there is a little information about the duplication of HER2/neu gene in gastric cancer using MLPA method. The present study aimed to investigate the frequency of mutations resulting from amplification of HER2/neu gene using MLPA technique in the patients with gastric cancer. Methods: This is a descriptive study which carried out on 60 samples of tissue block obtained from gastric cancer patients with endoscopic and surgical history between 1376-1391 years in Afzalipoor health centers to investigate the deletion and insertion mutations in HER2/neu. Results: The study population consisted of 73.3% females and 26.7% males with the mean age of 62.71. Overall, the frequency of the amplification of HER2/neu gene in the low and high levels was 10% and 3.3%, respectively. Conclusion: It seems that, the amplification frequency of HER2/neu gene in men is more than women. However, it is recommended to conduct further studies. %> http://jms.thums.ac.ir/article-1-264-en.pdf %P 45-38 %& 45 %! %9 Research %L A-10-143-2 %+ %G eng %@ 2716-9669 %[ 2015