RT - Journal Article T1 - Molecular Survey of Pathogen Species of Aspergillus Isolated from Diabetic Foot Lesion Using Nested PCR Method JF - thums-jms YR - 2014 JO - thums-jms VO - 1 IS - 4 UR - http://jms.thums.ac.ir/article-1-95-en.html SP - 25 EP - 32 K1 - Nested PCR - Sprzhylvs- foot Dyabty- culture AB - Background and Aim:  PCR has high sensitivity and specificity for the determination offungal DNA. It is also useful for rapid identification of the most common species of Aspergillus. The purpose of this study was to compare culture method with nested PCR method to determine Aspergillesion in diabetic foot patient in Imam Khomeini Hospital, Tehran. Material and Methods: Sixty-five cases of suspicious diabetic foot lesion Aspergillusis were examined under culture and molecular method. Aspergillus isolates were identified to genus level on the SGA. DNA extraction performed and PCR and Nested PCR reactions were done. Serum samples were collected and ELISA for anti bodies against all species were performed. Results were analyzed using ANOVA and P was considered significant at P   LA eng UL http://jms.thums.ac.ir/article-1-95-en.html M3 ER -