Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
Optimization of sono-nanocatalytic process using 𝛾-Fe2O3 for Penicilin antibiotic removal by response surface methodology
1
16
FA
Hadis
Hemmati
Health Promotion Research Center
hadis_hemati@yahoo.com
N
Edris
Bazrafshan
Health Promotion Research Center
ed_bazrafshan@yahoo.com
Y
Hossein
Kamani
Health Promotion Research Center
hadis_hemati@yahoo.com
N
Jafar
Mosafer
nanotechnology Research Center, School of Pharmacy
mosaferj901@gmail.com
N
Davoud
Balarak
Health Promotion Research Center
N
Ferdos
Kord Mostafapour
Health Promotion Research Center
N
Background & Aim: The pollution of water with pharmaceutical compounds can cause problems in the ecosystem. Antibiotics have special importance due to their inducing bacterial resistance. The aim of this study was to optimize the sono-nanocatalytic process using Fe₂O₃ for removal of Penicilin antibiotic by response surface methodology.
Methods: The study was based on the practical laboratory method in order to evaluate the effect of independent parameters such as pH, the dose of nanoparticles, reaction time, the initial concentration of the antibiotic and Frequency sound waves on the rate of penicillin removal. Chemical oxygen demand (COD) was selected to follow the performance for Penicilin antibiotic removal. In order to achieve the optimal experimental conditions, response surface methodology (RSM) model was designed and used.
Results: The results of data analysis showed that the catalyst dose and reaction time had greatest impact on the COD removal efficiency of Penicilin. Also, optimum removal conditions based on the analysis of variance (ANOVA) and model was achieved at pH, frequency of sono waves, initial concentration of the antibiotic, catalyst dose and reaction time equal 3, 35 KHz, 10 mg/l, 0.3 g/L and 53 min, respectively. Under these conditions, a COD removal efficiency equal to 95.51% was achieved.
Conclusion: The results of this study showed that the sono-nanocatalytic process in the presence 𝛾- Fe₂O₃ nanoparticles has a high efficiency on the COD removal (Penicilin antibiotic) from aqueous environments.
Fe2O3 nanoparticles, Penicilin antibiotic, Sonocatalytic process, Response Surface Methodology (RSM)
http://jms.thums.ac.ir/article-1-446-en.html
http://jms.thums.ac.ir/article-1-446-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
The Frequency of blaPER ، blaVEB، blaCTX-M، tetA and tetB genes among Acinetobacter baumannii strains isolated from hospitalizes patients in Tehran
17
25
FA
Omid
Azizi
1- Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Azizi-omid@outlook.com
Y
Fereshteh
Shahcheraghi
3- Department of Bacteriology, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
N
Background & Aim: Infections and outbreaks caused by multidrug-resistant Acinetobacter baumannii are prevalent and have been reported worldwide over the past twenty or more years. Beta-lactamase genes including blaPER, blaVEB and blaCTX-M confer resistance to beta-lactam antibiotics and tetA and tetB are responsible for resistance to tetracycline in such bacteria.
Methods: A total of 65 isolates of A. Baumannii from clinical samples were collected. Antimicrobial susceptibility testing was performed by the disk diffusion method according to the CLSI guideline and the presence of blaOXA-51 tetA, tetB, blaVEB, blaCTX and blaPER were screened via the polymerase chain reaction (PCR).
Results: The isolates were 100% resistant to gentamicin, ciprofloxacin, piperacillin, cefotaxime, ceftazidime and tetracycline. Resistance to minocycline and imipenem stood at 89% and 85%, respectively. All isolates were identified as multi-drug resistant (MDR). The genes tetA, tetB, blaVEB, blaCTX and blaPER were detected in 75.3%, 43%, 35.3%, 76.9% and 61.5% of isolates, respectively.
Conclusion: This study revealed the high prevalence of antimicrobial resistance genes amongst Acinetobacter baumannii and thus confirms the need for isolating and identifying them in clinical laboratory and hospital settings.
Acinetobacter, Antibiotic resistance, Beta-lactamase
http://jms.thums.ac.ir/article-1-443-en.html
http://jms.thums.ac.ir/article-1-443-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
Evaluating the Effects of Deregulated miRNAs by Human Papilloma Virus on Gene Expression Profiles of Squamous Cell Carcinomas of the Head and Neck
26
38
FA
Mohsen
Navari
Department of Virology, Pasteur Institute of Iran, Tehran, Iran
mohsen.navari@gmail.com
Y
Mostafa
Ibrahimi
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran. Iran
N
Milad
Mohammadoo-khorasani
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran. Iran
N
Arash
Arashkia
Department of Virology, Pasteur Institute of Iran, Tehran, Iran
maryamtatary@yahoo.com
N
Background and aim: Human Papilloma Virus plays an important role in some of human malignancies and causes alterations in normal expression levels of cellular microRNAs. In this paper, we evaluated the effects of such changes on Head and Neck Squamous Cell Carcinoma tumor samples at gene expression profile level.
Methods: in this descriptive-analytical study, gene expression profiles of 36 tumor samples were compared in two groups: with or without virus. Differentially expressed genes among the two groups were judged in terms of their ability in segregating the tumor samples and also their overlap with Gene Ontology Biological Function categories. Furthermore, using hierarchical clustering analysis and Gene Set Enrichment Analysis methods, the effect of confirmed cellular targets of 11 reported cellular microRNAs on the gene expression profiles of our samples was assessed.
Results: Unlike unsupervised methods, differentially expressed genes, including 47 and 7 unique induced and suppressed genes, respectively, discriminated perfectly the two sample sets in a hierarchical clustering analysis (P=0.0001). These genes were primarily engaged in regulation of cell cycle (FDR adjusted P≤0.05). Targets of induced cellular microRNAs were found enriched in virus-positive set (FDR adjusted P≤0.05). Among analyzed cellular miRNAs, hsa-miR-155-5p and hsa-miR-221-3p change the gene expression profile of tumor samples significantly (FDR adjusted P≤0.05).
Conclusion: deregulating expression levels of cellular microRNAs, HPV is capable of affecting the gene expression profiles of Head and Neck Squamous cell Carcinoma tumors. It is suggested to confirm the results of this study using experimental methods.
Human Papilloma Virus, Squamous cell carcinoma of the head and neck, microRNA, Gene Expression Profiling
http://jms.thums.ac.ir/article-1-452-en.html
http://jms.thums.ac.ir/article-1-452-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
AS1411 Aptamer-Mediated Targeted Drug Delivery of Doxorubicin-loaded PLGA Nanoparticles to Cancer Cells through Cell Surface Nucleolin
39
47
FA
Jafar
Mosafer
Mashhad University of Medical Sciences
Mosaferj901@gmail.com
Y
Edris
Bazrafshan
Zahedan University of Medical Sciences
ed_bazrafshan@yahoo.com
N
Background & Aim: Nucleolin is one of the most abundant proteins in the nucleolus that is overexpressed on the surface of the plasmic membrane of cancer cells. It has been suggested that nucleolin is a new and promising candidate for effective targeted active-targeted delivery of nanoparticles with anti-nucleolin AS1411 aptamer (hereafter Apt), as a single-strand DNA, into a variety of high nucleolin-expressing cancer cells compared to low nucleolin-expressing cell lines.
Methods: In this study, doxorubicin (Dox), as a chemotherapy drug with a fluorescence nature, was entrapped into the Poly (lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs). Next, these NPs were conjugated to Apt and the targeting ability of these Apt-NPs was investigated by flow cytometry and cytotoxicity analysis.
Results: As a result, more rapidly internalization of Apt-NPs into C26 and C6 cancer cells was verified compared with L929 as a low nucleolin-expressing cell line. Similarly, the Apt-NPs increased the cytotoxicity effect of Dox compared with NPs and free Dox solution alone.
Conclusion: We think that Apt-NPs, as a ligand, first bind to nucleolin and that the receptor-ligand complex is then incorporated into the cells through receptor-mediated endocytosis pathway. In conclusion, the Apt-NPs were found to be a promising targeted delivery system for therapeutic purposes.
Nucleolin, AS1411 aptamer, targeted delivery, PLGA, Doxorubicin
http://jms.thums.ac.ir/article-1-451-en.html
http://jms.thums.ac.ir/article-1-451-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
Evaluation of the Performance of Hospitals in Torbat Heydarieh City Based on the PABON LASSO Model and its Relative Comparison with National Standards
48
55
FA
Behnaz
Barati
Tehran University of Medical Sciences
bbarati491@gmail.com
N
Fereshteh
Farzianpour
Tehran University of Medical Sciences
farzianp@sina.tums.ac.ir
Y
Mohammad
Arab
Tehran University of Medical Sciences
arabmoha@tums.ac.ir
N
Abbas
Rahimi Froushani
Tehran University of Medical Sciences
rahimifo@tuma.ac.ir
N
Background and Aim: In the field of health, due to the limited resources and sensitivity of the subject of treatment, performance evaluation gains more importance. The main objective of this research was to evaluate the performance of two hospitals in Torbat Heydarieh city based on the PABON LASSO model and to compare the performance indicators with national standards during 2014-2015.
Methods: This descriptive-analytical cross-sectional study was carried out in two of the city's Razi Medical Training and Social Security hospitals. The data was collected from the medical records unit of the relevant hospitals. SPSS19 and EXCEL software were used to analyze the data and plot the PABON LASSO chart. One-sample t-test was used for comparative comparisons.
Results: The average Bed Occupancy Rate, Bed Turnover Rate and Average Length of Stay in the study period were 73.83, 131.33times, and 2.3 days, respectively. In the first year of the study, the medical training hospital located in District 1 and the Social Security Hospital in District 3; and in the second year, the medical training hospital between District 3 and 4 and the Social Security Hospital in District 2 were studied.
Conclusion: The mean of bed turnover and average length of stay in the two hospitals was far better than the national standard. However, it is suggested that in order to increase the utilization of hospital resources and to prevent wastage of the same through improved efficiency and performance, important performance indicators of the hospitals should be continually extracted and compared with other hospitals.
hospital, performance evaluation, Pabon Lasso Model, mean of bed occupancy, bed turnover rate, average length of stay
http://jms.thums.ac.ir/article-1-430-en.html
http://jms.thums.ac.ir/article-1-430-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
Comparison of antioxidant effects of hydro-alcoholic extract of Nigella sativa and Curcuma longa with Vitamin C on renal tissue oxidative stress parameters in rats
56
67
FA
Reza
Mohebbati
Mashhad University of Medical Sciences, Mashhad, Iran
mohebbatir931@mums.ac.ir
N
Saeid Reza
Ghanbarizadeh
Torbat Heydariyeh University of Medical Sciences
ghanbarizades1@thums.ac.ir
N
Farimah
Beheshti
Mashhad University of Medical Sciences, Mashhad, Iran
farimah.beheshty74@gmail.com
Y
Background and Aim: The aim of this study was to compare the effects of hydro-alcoholic extract of Curcuma longa (C.L E) and Nigella sativa (N.S E) with Vitamin C on renal-oxidative damage in rats.
Methods: 40 male Wistar rats were randomly divided into five groups (n=8) as follows: A Control group with plain drinking water and intervention groups including positive control groups with Vitamin C (100 mg/kg), the N.S E (200 mg/kg) group, the C.L E (1000 mg/kg) group and N.S E and C.L E group (receiving 200 and 1000 mg/kg, respectively), all dissolved in drinking water and fed during the 35 days of the experiment. At the end of this period, the renal tissues were removed and oxidation-reduction markers were investigated.
Results: N.S E (P <0.001), C.L E and vitamin C (P <0.01) decreased serum creatinine and BUN levels in comparison to the control group. Not only were the levels of total thiol higher in the Vitamin C (P <0.001), N.S E, C.L E (P <0.05) and N.S E and C.L E (P <0.01) groups compared to the control group, but also the superoxide dismutase (SOD) activity was more elevated in Vitamin C and N.S E and C.L E groups (P <0.01). Malondialdehyde (MDA) concentrations in the N.S E and C.L E, N.S E (P <0.001) and Vitamin C (P <0.05) groups were lower than those in the control group.
Conclusion: In the current study, it was found that N.S E and C.L E have a significant effect on the improvement of renal oxidative stress; which is comparable to Vitamin C. A higher synergistic effect of N.S E and C.L E suggested that they are more effective combined than when used separately.
Nigella sativa, Curcuma longa, Vitamin C, Oxidative stress
http://jms.thums.ac.ir/article-1-447-en.html
http://jms.thums.ac.ir/article-1-447-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
The effectiveness of group therapy based on Acceptance and Commitment (ACT) on loneliness and psychological adjustment in women after divorce
68
75
FA
Firouzeh
Najjari
Science and Research Branch, Islamic Azad University, Tehran, Iran
firoozeh.najjari@gmail.com
N
Anahita
Khodabakhshi Koolaee
Khatam University, Tehran, Iran
anna_khodabakhshi@yahoo.com
Y
Mohammad Reza
Falsafinejad
Allameh Tabataba'I University, Tehran, Iran
falsafinejad@yahoo.co.uk
N
Background & Aim: Psychological adjustment after divorce is one of the important related issues to divorce. This study aimed to investigate the effectiveness of group therapy based on acceptance and commitment to improving women's psychological adjustment and reducing loneliness after the divorce.
Methods: The study population consisted of all divorced women that were referred to counseling centers for psychological problems in Tehran by 2016. Among the clients of zone eight of city of Tehran, 30 women were selected and were assigned to two groups. This study is a semi experimental with pre-post-test and control group. The obtained data were analyzed by MANCOVA methods in software SPSS21.
Results: Based on the MANCOVA results, group therapy based on acceptance and commitment has been affective in improving Psychological adjustment and dimensions of abandonment, anger and grief (P=0.01).
Conclusion: Acceptance and commitment therapy guides clients to acceptance of suffering and commitment to goals instead of avoiding experience. Awareness of the thoughts and feelings of loneliness and being in the here and now are lead to improve the adjustment.
acceptance and commitment therapy, loneliness, Psychological adjustment, divorce.
http://jms.thums.ac.ir/article-1-459-en.html
http://jms.thums.ac.ir/article-1-459-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
5
3
2017
10
1
The Role of central neuroimmune activation in neuropathic pain and opioid Tolerance/Hyperalgesia
76
86
FA
Faranak
Jafari
School of Medical Sciences, Sabzevar University of medical Sciences, Sabzevar, Iran
faranakjafari@gmail.com
Y
Bahareh
Amin
Sabzevar University of medical Sciences, Sabzevar, Iran
amin.bahareh@gmail.com
N
Samad
Nazemi
Sabzevar University of medical Sciences, Sabzevar, Iran
samadnazemi@gmail.com
N
Omid
Gholami
Sabzevar University of medical Sciences, Sabzevar, Iran
N
Akbar
Pejhan
Sabzevar University of medical Sciences, Sabzevar, Iran
N
Common cellular and molecular mechanisms are not only involved in the development of neuropathic pain caused by neurological damage but also in the occurrence of the tolerance/hyperalgesia phenomenon caused by chronic use of opioids. It seems that the activation of the neuroimmune system in the brain and spinal cord is one of the most important mechanisms involved in the initiation and maintenance of neuropathic pains and reducing the antinociceptive effect of morphine after nerve injury. Plus, it also plays an important role in the development of tolerance/hyperalgesia due to chronic opioid consumption. Glial cells, especially microglia, are resident immune cells in the nervous system and get activated in response to many exogenous and endogenous factors. When activated, glial cells undergo structural and functional changes and can secrete various inflammatory factors such as IL1β, IL6 and TNFα. These changes increase the irritability and spontaneous firing of neurons, which play an important role in creating and maintaining neuropathic pain as well as reducing the analgesic effect of opioids and bringing about the onset of opioid tolerance/hyperalgesia phenomenon. In this review, we have tried to observe recent studies on the role of the neuroimmune system of the brain and spinal cord in the development of neuropathic pain and of opioid tolerance/hyperalgesia. In our view, a prevention of activation or a diminished activity of the neuroimmune system via appropriate drug compounds can be useful as a new strategy in the treatment of neuropathic pain and in the decrease of morphine tolerance/hyperalgesia, which will in turn result in an increase of the clinical efficacy of opioids.
Neuropathic pain, Hyperalgesia, Glia, morphine tolerance/hyperalgesia, opioids
http://jms.thums.ac.ir/article-1-454-en.html
http://jms.thums.ac.ir/article-1-454-en.pdf