Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
Optimization and comparison of three commercial carriers for efficient transfection into the peripheral blood mononuclear cells (PBMCs)
1
9
FA
Sepideh
Khatibi
Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
KHatibiS921@mums.ac.ir
N
Zeinab
Zare
Department of Tissue Engineering & Applied cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
zzarei19@yahoo.com
N
Seyed Hamid
Aghaee-Bakhtiari
Bioinformatics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
aghaeibh@mums.ac.ir
Y
Background & Aim: Peripheral Blood Mononuclear Cells (PBMCs) are the main components of the immune system, including lymphocytes and monocytes. These cells are being used in most immunological studies and therefore, isolation, culture and transfection of these cells are of great importance. The transfection of these cells is currently a key concern; this is because most common methods of transfection into the PBMCs are not well suited. The purpose of this study was to compare and optimize the effects of three commercial methods (PolyFect, Lipofectamine 2000 and Lipofectamine 3000) on PBMC transfection using PMAX-GFP vector.
Methods: PBMC cells were isolated from the peripheral blood by Ficole. Following the cell culture, the PMAX-GFP vector was transfected into the cells using the Polyfect, Lipopactamine 2000 and Lipopactamine 3000. After 24, 72 and 120 hours, the transfection rate for each method was evaluated by fluorescence microscope.
Results: Based on the obtained results, the level of transfection for PBMCs by Lipopactamine 2000 and Lipopactamine 3000 at all times was about 2-3%. The amount of transfection of these cells by the Polyfect after 120 hours was about 70%.
Conclusion: Generally, the efficiency of transfection of PBMCs s by Polyfect was probably the best in terms of increasing time, which introduces a new method for gene transfer into these cells. However, further studies are required to achieve the highest conversion efficiency of PBMC cells.
PBMC cells, Gene Delivery, Non-viral vector
http://jms.thums.ac.ir/article-1-533-en.html
http://jms.thums.ac.ir/article-1-533-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
Diagnosis of Diabetes using Artificial Neural Network and Neuro-Fuzzy approach
10
20
FA
Iman
Zabbah
Departement fo Electrical and Computer Engineering, University of Torbat Heydarieh, Iran
imanzabbah@gmail.com
Y
Asma
Eskandari
University of Torbat Heydarieh, Torbat Heydarieh, Iran
asma.eskandari@gmail.com
N
Zahra
Sardari
University of Torbat Heydarieh, Torbat Heydarieh, Iran
zahra.sardari@gmail.com
N
Abolfazl
Noghandi
University of Torbat Heydarieh, Torbat Heydarieh, Iran
abolfazlnoghandi@gmail.com
N
Background & Aim: A main problem in diabetes is its timely and accurate diagnosis. This study aimed at diagnosing diabetes using data mining methods.
Methods: The present study is an analytical investigation including 768 individuals with 8 attributes. Artificial neural networks and fuzzy neural networks were used to diagnose the diabetes. To achieve a real accuracy, the Kfold method was used to divide samples into training and test groups.
Results: The mean square errors in multilayer perceptron network (MLP), learning vector quantization and Nero fuzzy networks were 98.6%, 98.2% and 99.6%, respectively.
Conclusion: According to the results of this study, , data mining method can be effective in diagnosing diabetes. In this regard, both used methods are useful; however, higher precision was obtained following the use of Neuro-Fuzzy approach.
Diabetes, Artificial Neural Networks, Fuzzy Neural networks, Data mining
http://jms.thums.ac.ir/article-1-500-en.html
http://jms.thums.ac.ir/article-1-500-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
Investigating the effect of several determinative factors on gastric cancer morbidity in Aghbulagh Village, Meshgin Shahr –Ardabil Province: A case control study
21
28
FA
Fateme
Mehravar
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
freshtehnajafi@yahoo.com
N
Fereshteh
Najafi
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
freshtehnajafi@yahoo.com
N
malihe
khoramdad
Department of Epidemiology and Biostatistics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
freshtehnajafi@yahoo.com
N
Yaser
Taaraei
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
freshtehnajafi@yahoo.com
N
Mohammadali
Mansoornia
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
freshtehnajafi@yahoo.com
N
Koorosh
Holakoui
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
freshtehnajafi@yahoo.com
Y
Background & Aim: Gastric cancer is the most common gastrointestinal cancer in Iran, especially in the northwestern part of the country. The purpose of this study was to investigate the factors affecting gastric cancer in the village of Aghbolagh MeshginShahr, Ardebil.
Methods: This study was carried out in the village of Agbolagh located in Meshginshahr, Ardebil province in which gastric cancer was considered as a highly prioritized concern. Hence, a case-control study was designed on 21 patients with gastric cancer diagnosed during 2013-2015. This study included 63 control subjects who were randomly and purposefully selected from the adjacent villages and matched for age and sex. Odds ratio and logistic regression were calculated for probable risk factors.
Results: The mean age in the case group was 64.7 years and in control group was 61.1 years. In multivariate analysis, using logistic regression, educational level variables (OR = 0.38, CI = 0.15-0.88), family history (OR = 12.9, CI =2.5-72.5) and low fruit consumption (OR =3.06, = 0.49-9.89) were significantly different compared to control cases. Also, single-variable analysis revealed a significant difference for the.intervals between meals (OR =3.5, CI =1.2-9.9).
Conclusion: Given the high odds ratio for family history variables, genetic studies are recommended to identify the involved genes and their interactions with the environmental factors in this region.
Gastric cancer, stomach cancer, Case-control, Gastrointestinal Cancer, Community assessment
http://jms.thums.ac.ir/article-1-490-en.html
http://jms.thums.ac.ir/article-1-490-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
Investigation of fluoride in potable water resources in Torbat Heydariyeh City in 2016-17
29
37
FA
Mojtaba
Davoudi
Department of Environmental Health Engineering, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
davoudi85@gmail.com
N
Fateme
Barjasteh Askari
Department of Environmental Health Engineering, School of Health, Tehran University of Medical Sciences, Tehran, Iran
fatemeh.barjasteh@gmail.com
Y
Aminallah
Zarei
Department of Environmental Health Engineering, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
aminallahzarei@gmail.com
N
Mohammad
Afsharmoghadam
Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
mohammadafshar625@yahoo.com
N
Hossein
Ghasemzadeh
Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
hoseinghasemzade@gmail.com
N
Mahdi
Mojtabazadeh
Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
mojtabazadeh.m@yahoo.com
N
Background & Aim: Fluoride concentration in drinking water within the optimal range reduces dental caries and prevents dental fluorosis. This study was conducted to determine the level of fluoride in drinking water resources in Torbat Heydariyeh City in 2016-17.
Methods: This descriptive cross-sectional study was conducted on 22 wells used to supply drinking water of Torbat Heydariyeh City. A seasonal sample of one litre was withdrawn from each well and the fluoride concentration was measured using the SPADNS standard method with a DR 5000 spectrophotometer. Data were analyzed by SPSS version 11 software.
Results: The concentration of fluoride in the study area was measured in the range of 0 to 0.35 mg/L with an average of 0.23 mg/L. No significant seasonal changes were observed in the fluoride concentration. In addition, there was no significant relationship between fluoride concentration and well depth.
Conclusion: Considering the fact that observed fluoride concentrations were below the standard level, it is recommended to take measures to prevent dental caries due to fluoride deficiency such as distribution of mouthwash containing fluoride in schools and fluorination of drinking water.
Fluoride, Drinking Water, Dental Caries, Torbat Heydariyeh
http://jms.thums.ac.ir/article-1-470-en.html
http://jms.thums.ac.ir/article-1-470-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
Studying the knowledge and attitude of students of Torbat Heydariyeh University of Medical Sciences toward e-learning
38
47
FA
Hamide
Jafari
Department of Midwifery, School of Nursing and Midwifery, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
jafari.hamideh@yahoo.com
N
Eham
Azmoude
Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
azmoudehe@gmail.com
Y
Maryam
Ahour
Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
ahour8282@gmail.com
N
Background & Aim: Electronic learning as one of the new educational methods has the potential to enter the field of medical education and continuing education. Therefore, it is necessary to evaluate the level of knowledge and attitude of individuals towards this educational method in order to improve the quality and quantity level of universities. The purpose of this study was to determine the knowledge and attitude of students of Torbat Heydariyeh University of Medical Sciences towards e-learning in 2016.
Methods: In this descriptive-analytic study, 190 students of Torbat Heydariyeh University of Medical Sciences were assessed in 2016. The data collection tools included demographic, access and use of information technology, knowledge assessment and Mishra’s E-Learning Attitude Scale questionnaires. Data analysis was performed by means of SPSS software Version 16 using descriptive statistics and Mann-Whitney, Kruskal-Wallis and Spearman correlation tests.
Results: The mean score of knowledge and attitude of the subjects towards e-learning were 8.77 ± 4.35 (0 to 30) and 38.77 ± 6.87, respectively. There was also a significant relationship between gender, educational semester and the experience of passing courses in electronic format with the student's knowledge score.
Conclusion: Students of Torbat Heydariyeh University of Medical Sciences had a poor level of knowledge buta positive attitude toward e-learning. Therefore, it is recommended that, after providing e-learning platforms such as improving people's knowledge, this method be used alongside traditional learning methods.
E-learning, knowledge, attitude
http://jms.thums.ac.ir/article-1-495-en.html
http://jms.thums.ac.ir/article-1-495-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
The relationship between symptoms of paranoid, schizoid and schizotypal personality disorder whit impaired theory of mind
48
57
FA
mansor
beyrami
university of tabriz
dr.bayrami@yahoo.com
N
touraj
hashemi nosrat abad
university of tabriz
tourajhashemi@yahoo.com
N
nasrin
bahri sis
university of tabriz
nasrin.bahri70@gmail.com
Y
Background & Aim: theory of mind is one of the brain that facilitates social cognitive abilities or understanding of the social interaction. The aim of this study was to determine the relationship between personality disorder and paranoid, schizoid and schizotypal with impaired theory of mind..
Methods: For this purpose 210 students of Tabriz University were selected by random cluster sampling. Then students Questionnaire III clinical trial Millon and mind reading test through the eyes Baron-Cohen completed. For data analysis, structural equation modeling (SEM) was used.
Results: The results showed that the change theory of mind deficits with symptoms of paranoid personality disorder, schizoid and schizotypal is positive.
Conclusion: The results showed that people with paranoid personality disorder and schizotypal positive and significant relationship with impaired theory of mind. But the symptoms of schizoid personality disorder theory of mind deficits are not a meaningful role in explaining the changes.
: Theory of mind, paranoid personality disorder, schizoid personality disorder, schizotypal personality disorder
http://jms.thums.ac.ir/article-1-482-en.html
http://jms.thums.ac.ir/article-1-482-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
Investigation of the environmental health and safety status of primary schools in Torbat Heydariyeh city in 2016-2017
58
65
FA
Mohammad
Sarmadi
Department of Environmental Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
msarmadi2@gmail.com
Y
Edris
Bazrafshan
Department of Environmental Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
jahanarat1@thums.ac.ir
N
Amin Allah
Zarei
Department of Environmental Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
N
Fatemeh
Barjasteh Askari
Department of Environmental Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
barjastehfi@thums.ac.ir
N
Tahere
Jahanara
Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
N
Maryam
Tatatri
Tatarim1@thums.ac.ir
N
Background & Aim: School is one of the most important places for people’s mental development the effects of which can be observed through everyone’s educational and professional life. This study investigated the health and safety status in elementary schools of Torbat Heydariyeh City.
Methods: This descriptive cross-sectional study was carried out in 2016-17 on 68 elementary schools in Torbat Heydariyeh City, which were categorized based on gender and being governmental or non-governmental. Information was collected through field surveys and interviews with school officials and using a standard checklist provided by Ministry of Health. Descriptive data were analyzed using MS Office’s Excel version 2010 and statistical analysis was performed by SPSS version 22. Chi-square and Fisher's exact test were used to analyze the variable.
Results: Our results showed that based on the defined triple levels for schools, i.e. poor, moderate and desirable, the analyzed schools were categorized as moderate or desirable.-. Accordingly, 47.4% and 36.4%, of public and non-governmental schools were found to be in moderate level; and 52.6% and 63.6% of them were in desirable level, respectively. In general, we found no significant difference in the health status of the schools when analyzed based on the administration type (governmental and none) or gender (male vs. female).
Conclusion: Considering the presented results and the importance of elementary schools as the first post-family community for the students, the efforts of the institutions involved in education with the aim of establishing maximum health requirements are essential.
School, Environmental Health, Safety, Torbat Heydariyeh
http://jms.thums.ac.ir/article-1-433-en.html
http://jms.thums.ac.ir/article-1-433-en.pdf
Torbat Heydarieh University of Medical Sciences
Journal of Torbat Heydariyeh University of Medical Sciences
2716-9669
2716-9669
6
2
2018
9
1
The effects of common herbal extracts on epilepsy: a review article
66
73
FA
Hanieh
Sahebkar khorghi
Payame Noor University, Torbat Heydarieh, Iran
haniyehsahebkar@yahoo.com
N
Faranak
Jafari
Neuroscience Research Center, Department of Physiology, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
faranak jafari28@gmail.com
N
Azam
Ramezani
Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
N
Hassan
Azhdari-zarmehri
Neuroscience Research Center, Department of Physiology, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
hasan.azhdari@gmail.com
N
Masoumeh
Gholami
Neuroscience Research Center, Department of Physiology, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Gholamim3@thums.ac.ir
Y
Background & Aim: Epilepsy is one of the most common brain disorders following stroke, which affects about one percent of the world's population. Regarding the complications of chemical drugs and the resistance of some patients with epilepsy to these drugs, one of the most important approaches in treatment and prevention of epilepsy is the use of herbal extracts.
Methods: In this review study, Elsevier, Scopus, Science Direct and PubMed databases were searched for epilepsy, seizures, plant extracts and brain keywords from 1980 to 2018. Finally, 50 related articles were included.
Results: Animal studies have shown that many of these plants inhibit seizure activity in epilepsy through affecting GABA, glutamate or calcium channel receptors, sodium channels, chlorine channels and also by reducing the level of oxidative stress.
Conclusion: Regarding the economic issues and the adverse effects of chemical drugs, the use of herbal extracts with fewer complications, can be a good alternative for the treatment and prevention of epilepsy.
Epilepsy, Seizure, Plant extract, Brain
http://jms.thums.ac.ir/article-1-491-en.html
http://jms.thums.ac.ir/article-1-491-en.pdf