AU - J, Mosafer AU - H, Kamali AU - M, Tafaghodi AU - M, khajooee TI - Preparation and in-vitro evaluation of an in-situ gel forming of chitosan-based morphine formulation as an intranasaladministrationRunning title: An intranasal in-situ gel forming of morphine PT - JOURNAL ARTICLE TA - thums-jms JN - thums-jms VO - 9 VI - 3 IP - 3 4099 - http://jms.thums.ac.ir/article-1-1018-en.html 4100 - http://jms.thums.ac.ir/article-1-1018-en.pdf SO - thums-jms 3 ABĀ  - Background & Aim: Intranasal delivery through the epithelium of nose is an interesting and useful idea because it has a fast onset of effect with any metabolism in liver and digestive system, also it is user-friendly, noninvasive and without pain. In this study, we aimed to prepare and evaluate an in-situ gel forming of chitosan-based morphine formulation as an intranasal administration in vitro. Methods: In this project, the in-situ gel forming of morphine-loaded chitosan formulation was firstly prepared and finally its physicochemical properties was determined in order to achieve the technology of a gel-forming formulation for morphine nasal delivery. Results: The final prepared formulation was in the form of sol in room temperature; while, it was transmitted to the gel form at 37 °C and subsequently returned to the sol form after decreasing temperature. The obtained results of burst release test showed a sustained release of morphine from the polymeric matrix of chitosan. Additionally, the formulation showed that it could be sprayed in nostril with no toxicity. Conclusion: The biodegradable and biocompatible intranasal form of morphine could be gelled in the shortest possible time before leaving the nose at a temperature of 34 °C. Also, based on the release pattern of morphine, morphine could slowly induce its analgesic effects. CP - IRAN IN - Torbat Heydariyeh University of Medical Sciences LG - eng PB - thums-jms PG - 1 PT - Research YR - 2022