TY - JOUR T1 - The effect of Gingerol on reduction of oxidative stress-induced cytotoxicity and apoptosis in human adipose tissue and rat bone marrow -derived mesenchymal stem cells TT - کاهش سایتوتوکسیسیتی و آپوپتوز ناشی از استرس اکسیداتیو در سلول های بنیادی مزانشیمی مشتق از بافت چربی انسان و مغز استخوان موش صحرایی بوسیله جینجرول JF - thums-jms JO - thums-jms VL - 9 IS - 2 UR - http://jms.thums.ac.ir/article-1-931-en.html Y1 - 2021 SP - 1 EP - 11 KW - Gingerol KW - Oxidative stress KW - Antioxidant KW - Mesenchymal stem cells KW - Apoptosis KW - Cytotoxicity N2 - Background & Aim: Mesenchymal stem cells (MSCs) are a good candidate for regenerative medicine due to their differentiation and self-renewal properties. However, one of the leading problems is oxidative stress in the target tissue and apoptosis of transplanted stem cells before tissue repair. Pre-treatment of stem cells with antioxidants may make them resistant to oxidative stress. This study was conducted to investigate the antioxidant effect of gingerol on oxidative stress-induced apoptosis and cytotoxicity in mesenchymal stem cells derived from human adipose tissue and rat bone marrow. Methods: In the present research study, the human adipose tissue and rat bone marrow mesenchymal stem cells were incubated 4 and 6 hours for pretreatment with different concentrations of gingerol (50, 100, 200 and 400 mg / ml) ,then treated with 200 μM H2O2 for 2 hours. Cytotoxicity and apoptosis was analyzed by MTS kit and Annexin V-FITC/PI kit, respectively. Statistical analysis was accomplished by ANOVA and Duncan tests using FlowJo and SPSS 18 softwares. Results: Gingerol treatment with all concentrations increased the dose and time dependent bioavailability of human adipose tissue-derived mesenchymal stem cells It also decreased apoptosis in rat bone marrow mesenchymal stem cells in a dose- and time-dependent manner. Conclusion: Gingerolincreases the efficiency of mesenchymal stem cells in tissue regeneration by reducing of oxidative stress. M3 ER -