Journal of Torbat Heydariyeh University of Medical Sciences
مجله دانشگاه علوم پزشکی تربت حیدریه
jmsthums
Medical Sciences
http://jms.thums.ac.ir
1
admin
2716-9669
2716-9669
10.61882/jmsthums
fa
jalali
1402
4
1
gregorian
2023
7
1
11
1
online
1
fulltext
fa
اثر تمرین تناوبی با شدت بالا بر تغییرات آنزیم های آنتی اکسیدانی بافت کبد در موش های صحرایی نر تغذیه شده با غذای پرچرب
The effect of high-intensity interval training on changes in antioxidant enzymes of liver tissue in male rats fed with high-fat diet
تخصصي
Special
پژوهشي
Research
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">مصرف غذای پرچرب موجب چاقی شده و می­تواند در افزایش رادیکال­ های آزاد و کاهش فعالیت آنزیم ­های آنتی ­اکسیدانی نقش داشته باشد. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">عدم تعادل بین تولید </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">رادیکال­ های آزاد و دفاع </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">ضداکسایشی ممکن است به آسیب بافت کبدی منجـر شود. </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">این مطالعـه با هـدف ارزیابی اثر </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">تمـرین تناوبی با شدت بالا بر تغییـرات آنزیـم ­های آنتی ­اکسیدانی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> سوپراکسید دیسموتاز (</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">SOD</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">) و گلوتاتیون پراکسیداز (</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">GPX</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> بافت کبد </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">موش ­های صحرایی تغذیه ­شده با غذای پرچرب اجرا شد.</span></span></span></span></span><br>
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">روش ­ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> در این مطالعه تجربی، 21 سر موش صحرایی نر بالغ نژاد ویستار به­طور تصادفی به سه گروه کنترل تغذیه ­شده با غذای نرمال، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">کنترل </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">تغذیه­ شده با غذای پرچرب و تغذیه ­شده با غذای پرچرب+ تمرین تناوبی با شدت بالا تقسیم شدند. حیوانات هر سه گروه به مدت 23 هفته با رژیم غذایی خاص خود تغذیه شدند. گروه تمرینی ضمن دریافت رژیم پرچرب 8 هفته پایانی پروتکل تمرین تناوبی شدید را سه جلسه در هفته اجرا کردند. در پایان مداخله، سطح آنزیم ­های </span></span></span>SOD<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و </span></span></span>GPX<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> در بافت کبد به روش رنگ ­سنجی آنزیمی اندازه­ گیری شد.</span></span></span></span></span><br>
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتایج:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> سطح آنزیم </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">GPX</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> کبدی در گروه تغذیه ­شده با غذای پرچرب+ تمرین تناوبی با شدت بالا در مقایسه با گروه ­های کنترل تغذیه ­شده با غذای نرمال ( 0/003=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">P</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و کنترل </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">تغذیه ­شده با غذای پرچرب (0/001=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">P</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">افرایش معناداری را نشان داد. با این ­وجود، تغییر معناداری در سطح </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">آنزیم </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">SOD</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> کبدی گروه ­های </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">مورد مطالعه مشاهده نشد </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">(0/153=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">P</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">).</span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Nazanin"">نتیجه ­گیری:</span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra""> نتایج این پژوهش بیانگر، آثار ضداکسایشی تمرین تناوبی با شدت بالا در بافت کبـدی موش ­هـای صحرایی تغذیه ­شده با </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">غذای پرچرب است</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">.</span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span style="font-size:11.5pt">Background & Aim</span></i></b><i><span style="font-size:11.5pt">:</span></i> <span lang="EN" style="font-size:11.5pt">Consuming high-fat </span><span style="font-size:11.5pt">diet</span><span lang="EN" style="font-size:11.5pt"> causes obesity and can play a role in increasing free radicals and reducing the activity of antioxidant enzymes. Imbalance between free radicals</span> <span lang="EN" style="font-size:11.5pt">production and antioxidant defense may lead to liver tissue damage. </span><span style="font-size:11.5pt">This study evaluates the effects high intensity interval training (HIIT) on alteration of hepatic antioxidant enzymes in rats<b> </b>fed with high-fat diet.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span style="font-size:11.5pt">Methods</span></i></b><b><span style="font-size:11.5pt">:</span></b><span style="font-size:11.5pt"> In this experimental study, 21 male Wistar rats were randomly divided into three groups: control </span><span lang="EN" style="font-size:11.5pt">fed with normal </span><span style="font-size:11.5pt">diet (n=7), control </span><span lang="EN" style="font-size:11.5pt">fed with </span><span style="font-size:11.5pt">high-fat diet (n=7), and </span><span lang="EN" style="font-size:11.5pt">fed with </span><span style="font-size:11.5pt">high-fat diet+ HIIT (n=7). </span><span lang="EN" style="font-size:11.5pt">The animals of all three groups were fed with their own diet for 23 weeks.</span> <span lang="EN" style="font-size:11.5pt">The training group while receiving a high-fat diet, in the final 8 weeks performed the </span><span style="font-size:11.5pt">HIIT</span><span lang="EN" style="font-size:11.5pt"> protocol three times a week</span><span style="font-size:11.5pt">. </span><span lang="EN" style="font-size:11.5pt">At the end of the intervention, SOD and GPX enzymes </span><span style="font-size:11.5pt">in liver tissue </span><span lang="EN" style="font-size:11.5pt">were </span><span style="font-size:11.5pt">assessed</span><span lang="EN" style="font-size:11.5pt"> by enzyme colorimetric method.</span><span style="font-size:11.5pt"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span style="font-size:11.5pt">Results</span></i></b><b><span style="font-size:11.5pt">: </span></b><span style="font-size:11.5pt">The results showed that liver </span><span lang="EN" style="font-size:11.5pt">GPX enzyme levels in the fed with </span><span style="font-size:11.5pt">high-fat</span><span lang="EN" style="font-size:11.5pt"> diet+ HIIT group increased significantly compared to the </span><span style="font-size:11.5pt">control </span><span lang="EN" style="font-size:11.5pt">fed with normal </span><span style="font-size:11.5pt">diet </span><span lang="EN" style="font-size:11.5pt">(P=0.003) and </span><span style="font-size:11.5pt">control </span><span lang="EN" style="font-size:11.5pt">fed with </span><span style="font-size:11.5pt">high-fat diet </span><span lang="EN" style="font-size:11.5pt">(P=0.001), but hepatic SOD enzyme levels did not change significantly in the three groups</span><span style="font-size:11.5pt"> (P=0.153).</span></span></span></span></span><br>
<b><i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Conclusion</span></span></span></i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">:</span></span></span></b><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""> The findings of this study imply that, </span></span></span><span lang="EN" style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">HIIT </span></span></span><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">presents an antioxidant stress effect on liver tissue in the rats<b> </b>fed with high-fat diet.</span></span></span>
سوپراکسید دیسموتاز, گلوتاتیون پراکسیداز, رژیم غذایی, تمرین تناوبی با شدت بالا, موش صحرایی
Superoxide dismutase, Glutathione peroxidase, Diet, High intensity interval training, Rat.
13
24
http://jms.thums.ac.ir/browse.php?a_code=A-10-201-3&slc_lang=fa&sid=1
Havasali
R
راضیه
هواسعلی
havasalir1362@gmail.com
100319475328460012897
100319475328460012897
No
Islamic Azad University Branch of Ilam
دانشگاه آزاد اسلامی واحد ایلام
Taheri Kalani
A
عبدالحسین
طاهری کلانی
htaheriedu@gmail.com
100319475328460012898
100319475328460012898
Yes
Islamic Azad University Branch of Ilam
دانشگاه آزاد اسلامی واحد ایلام
Fatahi
F
فردین
فتاحی
fatahi.fardin@yahoo.com
100319475328460012899
100319475328460012899
No
Islamic Azad University Branch of Ilam
دانشگاه آزاد اسلامی واحد ایلام