Journal of Torbat Heydariyeh University of Medical Sciences
مجله دانشگاه علوم پزشکی تربت حیدریه
jmsthums
Medical Sciences
http://jms.thums.ac.ir
1
admin
2716-9669
2716-9669
10.61882/jmsthums
fa
jalali
1402
6
1
gregorian
2023
9
1
11
2
online
1
fulltext
fa
مقایسه اثر عصاره هیدروالکلی و فلاونوئید تام برگ گیاه حرا (Avicennia marina) بر میزان Bax، Bcl-2، Caspase-9 و p53 در بافت تخمدان موشهای صحرایی دیابت نوع یک
Comparison of the effect of hydroalcoholic extract and flavonoid of Avicennia marina leaves on Bax, Bcl2, Caspase-9 and p53 in ovarian tissue of type 1 diabetic rats
تخصصي
Special
پژوهشي
Research
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">گیاه حرا دارای خواص آنتی­ اکسیدانی و هیپوگلیسمیک می باشد. فلاونوئید­های گیاه حرا از تشکیل رادیکال­های آزاد اکسیژن جلوگیری می­کند بنابراین تنش اکسیداتیو ناشی از بیماری های مختلف از جمله دیابت را مهار می نمایند. هدف از این مطالعه مقایسه اثر عصاره هیدروالکلی و فلاونوئید تام برگ گیاه حرا بر میزان </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Bax</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Bcl-2</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Caspase-9</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و پروتئین </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">p53</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> در بافت تخمدان موش­های صحرایی دیابت نوع یک می­باشد.</span></span></span></span></span><br>
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">روش­ ها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">در این مطالعه تجربی </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">42</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> سر موش صحرایی ماده نژاد ویستار به 6 گروه­ مساوی سالم، کنترل دیابتی، دو گروه دیابتی تحت تیمار با </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">عصاره هیدروالکلی اندام هوایی حرا (</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">50 و 100</span></span></span> mg/Kg<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">) و </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">دو گروه دیابتی تحت تیمار با </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">فلاونوئید تام اندام هوایی حرا (</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">50 و 100</span></span></span> mg/Kg<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">) به طور تصادفی ساده </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">تقسیم شدند. در پایان دوره درمان میزان </span></span></span>Bax<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، </span></span></span>Bcl-2<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، </span></span></span>Caspase-9<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و </span></span></span>p53<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> در بافت تخمدان توسط روش الایزا سنجش شد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span><br>
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتایج:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> با توجه به نتایج به­ دست آمده، میزان</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Bax </span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Caspase-9</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">p53</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> در بافت تخمدان موش­ های دیابتی تحت تیمار با غلظت­های 50 و 100 </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">mg/Kg</span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">فلاونوئید حرا در مقایسه با گروه کنترل دیابتی به­ صورت وابسته به دوز تزریقی</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> به­ </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">طور معنی­ داری کاهش و میزان </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Bcl-2</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> به­ طور معنی­ داری افزایش یافت (05/0></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">p</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">). میزان</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Bax </span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Caspase-9</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">p53</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> در بافت تخمدان گروه تحت تیمار با غلظت 100 </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">mg/Kg</span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">عصاره هیدروالکلی در مقایسه با گروه کنترل دیابتی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">به­ </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">طور معنی­ داری کاهش و میزان </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">Bcl-2</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> به­ طور معنی­ داری افزایش یافت (05/0></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">p</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">). در مقایسه بین فلاوونوئید کل و عصاره هیدروالکلی در غلظت 50 اختلاف معنی­ داری مشاهده نشد ولی در غلظت 100، فلاونوئید تام نسبت به عصاره هیدروالکلی تغییرات معنی داری را در پارامترهای مورد بررسی ایجاد نمود (05/0<</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">p</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">).</span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Nazanin"">نتیجه­ گیری:</span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra""> فلاونوئید تام برگ </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">در مقایسه با </span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">عصاره هیدروالکلی حرا </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">در کاهش آپوپتوزیس و پروتئین </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif">p53</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra""> در بافت تخمدان </span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">موش­های دیابتی نوع یک</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">می تواند موثرتر باشد</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">.</span></span></span>
<pre style="text-align:justify">
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><i><span style="font-size:11.5pt">Background & Aim</span></i></b><i><span style="font-size:11.5pt">:</span></i> <i><span style="font-size:11.5pt">Avicennia marina</span></i><i> </i><span lang="EN" style="font-size:11.5pt">plant has antioxidant and hypoglycemic properties.<b> </b>The flavonoids of the </span><i><span style="font-size:11.5pt">Avicennia marina</span></i><span lang="EN" style="font-size:11.5pt"> plant prevent the formation of oxygen free radicals; therefore,</span> <span lang="EN" style="font-size:11.5pt">it can inhibit the oxidative stress caused by various diseases, including diabetes.</span> <span style="font-size:11.5pt">This study aimed to investigate the effects of</span> <span style="font-size:11.5pt">hydroalcoholic extract and flavonoid of mangrove (<i>Avicennia marina</i>) leaves on Bax, Bcl<sub>2</sub>, Caspase-9 and p53 in ovarian tissue of type 1 diabetic rats</span><span dir="RTL" lang="AR-SA" style="font-size:11.5pt"><span style="font-family:"B Nazanin"">.</span></span><span style="font-size:11.5pt"></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><i><span style="font-size:11.5pt">Methods</span></i></b><b><span style="font-size:11.5pt">:</span></b><span style="font-size:11.5pt"> In this experimental study, 42 rats were randomly divided into 6 groups:</span> <span style="font-size:11.5pt">control, non-treated diabetic and 2 leaf total flavonoid -treated diabetic groups (50, 100 mg/ml, and 2 hydroalcoholic extract treated diabetic groups (50, 100 mg/ml)</span><span dir="RTL" lang="AR-SA" style="font-size:11.5pt"><span style="font-family:"B Nazanin"">.</span></span> <span lang="EN" style="font-size:11.5pt">At the end of the treatment period, Bax, Bcl<sub>2</sub>, Caspase-9 and p53 in ovarian tissue was measured by ELISA method.</span><span style="font-size:11.5pt"></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><i><span style="font-size:11.5pt">Results</span></i></b><b><span style="font-size:11.5pt">: </span></b><span lang="EN" style="font-size:11.5pt">According to the results, Bax, Caspase-9 and p53 in the ovarian tissue of diabetic rats treated with concentrations of 50 and 100 mg/Kg of total flavonoid of </span><i><span style="font-size:11.5pt">Avicennia marina</span></i><span lang="EN" style="font-size:11.5pt"> compared to the diabetic control group significantly decreased in a dose-dependent manner also Bcl<sub>2</sub> increased significantly.<b> </b>The amount of</span> <span lang="EN" style="font-size:11.5pt">Bax, Caspase-9 and p53 in the ovarian tissue of the group treated with a concentration of 100 mg/Kg hydro alcoholic extract significantly decreased and the amount of Bcl-2 increased significantly compared to the diabetic control group.<b> </b>In the comparison between total flavonoid and hydro alcoholic extract at a concentration of 50, no significant difference was observed, but at a concentration of 100, total flavonoid compared to the hydroalcoholic extract caused significant changes in the investigated parameters</span><span style="font-size:11.5pt">.</span></span></span></span></span>
<b><i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif">Conclusion</span></span></span></i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif">:</span></span></span></b> <span lang="EN" style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif">Total leaf flavonoid of </span></span></span><i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif">Avicennia marina</span></span></span></i><span lang="EN" style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"> is more effective in reducing apoptosis and p53 protein in ovarian tissue of type 1 diabetic rats compared to </span></span></span><i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif">Avicennia marina</span></span></span></i><span lang="EN" style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"> hydro alcoholic extract.</span></span></span>
</pre>
گیاه حرا, آپوپتوزیس, دیابت, بافت تخمدان,P53, موش صحرایی
Avicennia marina, apoptosis, Diabetes, ovarian tissue, p53, Rat
46
55
http://jms.thums.ac.ir/browse.php?a_code=A-10-1347-1&slc_lang=fa&sid=1
Rahbarian
R
راهله
رهباریان
ra_rahbarian@yahoo.com
100319475328460013220
100319475328460013220
Yes
Payame Noor University, Tehran
دانشگاه پیام نور، تهران
Ataei
Gh
غزال
عطایی
ahvaz.ghazal@yahoo.com
100319475328460013221
100319475328460013221
No
Payame Noor University, Tehran
دانشگاه پیام نور، تهران