Journal of Torbat Heydariyeh University of Medical Sciences
مجله دانشگاه علوم پزشکی تربت حیدریه
jmsthums
Medical Sciences
http://jms.thums.ac.ir
1
admin
2716-9669
2716-9669
10.61882/jmsthums
fa
jalali
1402
4
1
gregorian
2023
7
1
11
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online
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fulltext
fa
بررسی استرس اکسیداتیو و ژن BDNF در هیپوکامپ موش های صحرایی باردار تیمار شده با اتانول و تاثیر آن بر تعداد و وزن فرزندان
Investigation of Oxidative Stress and BDNF Gene in the Hippocampus of Pregnant Rats Treated with Ethanol and its Effect on the Number and Weight of Offspring
تخصصي
Special
پژوهشي
Research
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b><b> </b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">مصرف الکل در دوران بارداری باعث آسیب به مادر و جنین در حال تکامل</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> بخصوص</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> سیستم عصبی مرکزی می­شود.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">ایجاد استرس اکسیداتیو، فعال شدن مسیرهای مرگ سلولی و مداخله در بیان فاکتورهای نوروتروفیک جزو اختلالات حاصل از الکل می­باشد. این مطالعه با هدف بررسی ارتباط بین استرس اکسیداتیو و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">و میزان بیان ژن </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">BDNF</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> هیپوکامپ موشهای صحرایی باردار تیمار شده با اتانول و تاثیر آن بر تعداد و وزن فرزندان انجام شد.</span></span></span></span></span><br>
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">روش ­ها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">موش ­های صحرایی باردار به پنج گروه شش­ تایی تقسیم شدند: گروه کنترل (فقط آب مقطر) و چهار گروه تیمار که اتانول (20 درصد، 4/5 گرم/کیلوگرم) را</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">به صورت گاواژ دهانی به ترتیب در 5 روز اول، دهه اول بارداری، دهه دوم بارداری و کل دوران بارداری، دریافت نمودند. مادران در دوران بارداری و فرزندان پس از تولد وزن شدند. مادران</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">سه هفته</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> پس از زایمان طبق اصول اخلاقی قربانی شدند و بافت هیپوکامپ مغز آنها استخراج شد. فعالیت گلوتاتیون پراکسیداز (</span></span></span>GPx<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">)، سوپراکسید دسموتاز (</span></span></span>SOD<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">) و مالون دی­ آلدئید (</span></span></span>MDA<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">) در هیپوکامپ نمونه­ ها از طریق اسپکتروفتومتری ارزیابی شد. میزان بیان ژن </span></span></span>BDNF<span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> با روش </span></span></span>RT-PCR<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> اندازه­گیری شد. برای تحلیل داده ­ها از</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> نرم ­افزارهای</span></span></span> SPSS<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، </span></span></span>Excel<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">آزمون­ های آماری</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">آنالیز واریانس یک­ طرفه</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و توکی استفاده شد.</span></span></span></span></span><br>
<span style="line-height:115%"><span style="tab-stops:.05pt 1.7pt"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتایج:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">تجویز اتانول به موش­ های باردار تاثیری بر وزن مادران نداشت، اما موجب کاهش تعداد و وزن فرزندان، افزایش میزان </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">MDA</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، افزایش فعالیت </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">SOD</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">، کاهش فعالیت </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">GPx</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> و بیان </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%">BDNF</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""> در مقایسه با گروه کنترل شد</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra"">.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Nazanin"">نتیجه­ گیری:</span></span></span></b> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Mitra"">اتانول بیشترین اثرات نامطلوب خود را در گروه­های تحت تیمار در دهه ­ی دوم بارداری و کل دوره ­ی بارداری موش­ های صحرایی مادر داشته است.</span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span style="font-size:11.5pt">Background & Aim</span></i></b><i><span style="font-size:11.5pt">:</span></i> <span lang="EN" style="font-size:11.5pt">alcohol consumption during pregnancy causes damage to the mother and the developing fetus, especially the central nervous system. Oxidative stress, activation of cell death pathways and intervention in the expression of neurotrophic factors are among the disorders caused by alcohol. The aim of this study is to investigate the relationship between oxidative stress and BDNF gene expression in pregnant rats treated with ethanol and its effect on the number and weight of offspring.</span><span style="font-size:11.5pt"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span style="font-size:11.5pt">Methods</span></i></b><b><span style="font-size:11.5pt">:</span></b> <span lang="EN" style="font-size:11.5pt">Pregnant rats were divided into five groups of six: control group (distilled water only) and four treatment groups that received ethanol (20%, 4.5 g/kg) by oral gavage, respectively. They received the first 5 days, the first decade of pregnancy, the second decade of pregnancy and the entire period of pregnancy. Mothers were weighed during pregnancy and children were weighed after birth. Mothers were ethically sacrificed after giving birth and their hippocampal brain tissue was extracted. The activities of glutathione peroxidase (GPx), superoxide desmutase (SOD) and malondialdehyde (MDA) in the hippocampus of the samples were evaluated by spectrophotometry. BDNF gene expression was measured by RT-PCR method. SPSS, Excel and one-way analysis of variance and Tukey statistical tests were used for data analysis.</span><span style="font-size:11.5pt"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span style="font-size:11.5pt">Results</span></i></b><b><span style="font-size:11.5pt">: </span></b><span lang="EN" style="font-size:11.5pt">The administration of ethanol to pregnant rats did not affect the weight of the mothers, but it caused a decrease in the number and weight of offspring, an increase in MDA, an increase in SOD activity, a decrease in GPx activity and a decrease in BDNF expression compared to the control group.</span><span style="font-size:11.5pt"></span></span></span></span></span><br>
<b><i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Conclusion</span></span></span></i><span style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">:</span></span></span></b> <span lang="EN" style="font-size:11.5pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif"">Ethanol had the most adverse effects in the treated groups in the second decade of pregnancy and the entire pregnancy period of mother rats.</span></span></span>
اتانول, استرس اکسیداتیو, فاکتور رشد مشتق از مغز, بارداری, موش صحرایی
Ethanol, Oxidative Stress, BDGF, Pregnancy, Rat.
1
12
http://jms.thums.ac.ir/browse.php?a_code=A-10-1353-1&slc_lang=fa&sid=1
Shaabani Ghahremanlo
M
موسی
شعبانی قهرمانلو
Mousashabani007@gmail.com
100319475328460012893
0009-0007-4077-6339
No
Islamic Azad University, Damghan
دانشگاه آزاد واحد دامغان
Vaezi
Gh
غلامحسن
واعظی
Gh.vaezi@yahoo.com
100319475328460012894
100319475328460012894
No
Islamic Azad University, Damghan
دانشگاه آزاد واحد دامغان
Hojati
V
ویدا
حجتی
vida.hojati@gmail.com
100319475328460012895
100319475328460012895
Yes
Islamic Azad University, Damghan
دانشگاه آزاد واحد دامغان
Sharafi
Sh
شهرام
شرفی
Shahramsharafi2003@yahoo.com
100319475328460012896
100319475328460012896
No
Islamic Azad University, Damghan
دانشگاه آزاد واحد دامغان