Antibacterial activity of Fe2O3-nickel nanoparticles against drug resistant

Siasi, Elham; Taheri, Fatemeh; Takbiri, Laya

Volume 14, Issue 2 (6-2026) jmsthums 2026, 14(2): 12-26 | Back to browse issues page

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Siasi E, Taheri F, Takbiri L. Antibacterial activity of Fe2O3-nickel nanoparticles against drug resistant. jmsthums 2026; 14 (2) :12-26
URL: http://jms.thums.ac.ir/article-1-1464-en.html

Antibacterial activity of Fe2O3-nickel nanoparticles against drug resistant

Elham Siasi ^*¹

, Fatemeh Taheri¹

, Laya Takbiri¹

1- Department of Microbiology, NT.C., Islamic Azad University, Tehran, Iran

Abstract: (9 Views)

Background & Aim: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that can cause nosocomial infections and has a high capacity to develop antibiotic resistance. This study aimed to investigate the antibacterial effect of green-synthesized Fe2O3-nickel nanoparticles against drug-resistant Pseudomonas aeruginosa.
Methods: In this descriptive-analytical study, 50 clinical isolates of Pseudomonas aeruginosa were collected from patients hospitalized at Baqiyatallah Hospital in Tehran, based on the Cochran formula. The isolates were recovered and phenotypically identified using biochemical tests. Antibiotic susceptibility patterns were determined using the disk diffusion method according to CLSI standards, and the minimum inhibitory concentration (MIC) of imipenem was determined. Following DNA extraction, the presence of imipenem-resistance genes was investigated using PCR. Fe2O3-nickel nanoparticles were prepared using a green synthesis method, and their antibacterial activity was evaluated by determining the MIC.
Results: The highest resistance rates among the isolated Pseudomonas aeruginosa strains were reported against ampicillin (50%), imipenem (52%), and piperacillin–tazobactam (50%). PCR analysis revealed that 26 isolates carried the imipenem-resistance gene. The MIC of imipenem was greater than 8 μg/mL, while that of the iron–nickel nanoparticle extract was greater than 64 μg/mL. The minimum inhibitory concentration for bacterial growth using these nanoparticles differed significantly from that observed with antibiotics(p < 0.001).
Conclusion: Based on the findings of this study, green-synthesized iron–nickel nanoparticles may exhibit effective antibacterial activity against drug-resistant strains of Pseudomonas aeruginosa and have therapeutic potential. Further studies are recommended to confirm these findings.

Keywords: Pseudomonas Aeruginosa, Green Synthesis, Nanoparticle, Antibacterial Property, Gene

Full-Text [PDF 793 kb] (9 Downloads)

Type of Study: Research | Subject: Special
Received: 2026/02/19 | Accepted: 2026/05/30 | Published: 2026/10/19

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