Volume 13, Issue 4 (12-2025)
jmsthums 2025, 13(4): 26-36 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ahmadinejad S, Kazeminasab F. Effect of high-intensity interval training on hepatic CPT1A and SREBP-1c protein levels in male wistar rats. jmsthums 2025; 13 (4) :26-36
URL: http://jms.thums.ac.ir/article-1-1434-en.html
URL: http://jms.thums.ac.ir/article-1-1434-en.html
1- Department of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran
Abstract: (24 Views)
Background & Aim: Lipolysis and hepatic lipogenesis can help improve insulin resistance by regulating lipid metabolism and glucose. Exercise, as an effective strategy, plays an important role in increasing of CPT1a and reducing of SREBP1c protein levels and preventing complications of type 2 diabetes and obesity. Therefore, the aim of the present study was to investigate the effect of high-intensity interval training on CPT1a and SREBP1c protein levels in the liver tissue of male Wistar rats.
Methods: In this study, 12 Wistar rats were selected. The rats were randomly divided into two exercise groups and a control group. The control group was fed a standard diet until the end of the study. The exercise group performed aerobic exercise for 10 weeks according to a high-intensity interval training (HIIT) protocol. 48 hours after the last exercise session, the rats were anesthetized and liver tissue was extracted to examine CPT1a and SREBP1c protein levels. The protein levels were quantified using the Western blot technique. Comparison of the two groups was performed using independent t-test, considering the significance level (p<0.05) and using SPSS version 19 software.
Results: The results of the study indicated signs of intracellular fat accumulation (steatosis), ballooned cells, and irregular hepatocyte structure compared to the exercise group. Also, hepatocyte density was maintained in the exercise group compared to the control group, and no signs of obvious tissue damage were observed. The results showed that 10 weeks of HIIT significantly reduced the levels of SREBP1c (p=0.04), and significantly increased the levels of CPT1a protein in exercise rats compared to the control rats (p=0.02).
Conclusion: The results of the study indicate that HIIT has positive effects on improving liver metabolism by reducing lipogenesis and increasing hepatic lipolysis, and prevents the harmful effects of type 2 diabetes and obesity.
Methods: In this study, 12 Wistar rats were selected. The rats were randomly divided into two exercise groups and a control group. The control group was fed a standard diet until the end of the study. The exercise group performed aerobic exercise for 10 weeks according to a high-intensity interval training (HIIT) protocol. 48 hours after the last exercise session, the rats were anesthetized and liver tissue was extracted to examine CPT1a and SREBP1c protein levels. The protein levels were quantified using the Western blot technique. Comparison of the two groups was performed using independent t-test, considering the significance level (p<0.05) and using SPSS version 19 software.
Results: The results of the study indicated signs of intracellular fat accumulation (steatosis), ballooned cells, and irregular hepatocyte structure compared to the exercise group. Also, hepatocyte density was maintained in the exercise group compared to the control group, and no signs of obvious tissue damage were observed. The results showed that 10 weeks of HIIT significantly reduced the levels of SREBP1c (p=0.04), and significantly increased the levels of CPT1a protein in exercise rats compared to the control rats (p=0.02).
Conclusion: The results of the study indicate that HIIT has positive effects on improving liver metabolism by reducing lipogenesis and increasing hepatic lipolysis, and prevents the harmful effects of type 2 diabetes and obesity.
Type of Study: Research |
Subject:
Special
Received: 2025/08/23 | Accepted: 2025/11/25 | Published: 2026/02/9
Received: 2025/08/23 | Accepted: 2025/11/25 | Published: 2026/02/9
References
1. Jeon YG, Kim YY, Lee G, Kim JB. Physiological and pathological roles of lipogenesis. Nature Metabolism. 2023;5(5):735-59. [DOI:10.1038/s42255-023-00786-y]
2. Grabner GF, Xie H, Schweiger M, Zechner R. Lipolysis: cellular mechanisms for lipid mobilization from fat stores. Nature metabolism. 2021;3(11):1445-65. [DOI:10.1038/s42255-021-00493-6]
3. Yao Y, Zhang A, Zhang Z, Liu Z, Chua T-S, Sun M. Cpt: Colorful prompt tuning for pre-trained vision-language models. AI Open. 2024;5:30-8. [DOI:10.1016/j.aiopen.2024.01.004]
4. Schlaepfer IR, Joshi M. CPT1A-mediated fat oxidation, mechanisms, and therapeutic potential. Endocrinology. 2020;161(2):bqz046. [DOI:10.1210/endocr/bqz046]
5. Min C, Lei Z, Siyu C, Yaqian Q, Jingquan S. Acute and chronic effects of high-intensity interval training (HIIT) on postexercise intramuscular lipid metabolism in rats. Physiological Research. 2021;70(5):735. [DOI:10.33549/physiolres.934722]
6. Abu-Elheiga L, Matzuk MM, Abo-Hashema KA, Wakil SJ. Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2. Science. 2001;291(5513):2613-6. [DOI:10.1126/science.1056843]
7. Su F, Koeberle A. Regulation and targeting of SREBP-1 in hepatocellular carcinoma. Cancer and Metastasis Reviews. 2024;43(2):673-708. [DOI:10.1007/s10555-023-10156-5]
8. Ferré P, Phan F, Foufelle F. SREBP-1c and lipogenesis in the liver: an update. Biochemical journal. 2021;478(20):3723-39. [DOI:10.1042/BCJ20210071]
9. Wu B, Zhang Z, Xu C, Zhao J. Aerobic exercise improved liver steatosis by modulating miR-34a-mediated PPARα/SIRT1-AMPK signaling pathway. 2025. [DOI:10.21203/rs.3.rs-6207434/v1]
10. Kalaki-Jouybari F, Shanaki M, Delfan M, Gorgani-Firouzjae S, Khakdan S. High-intensity interval training (HIIT) alleviated NAFLD feature via miR-122 induction in liver of high-fat high-fructose diet induced diabetic rats. Archives of physiology and biochemistry. 2020;126(3):242-9. [DOI:10.1080/13813455.2018.1510968]
11. Eftekharzadeh M, Atashak S, Azarbayjani MA, Moradi L, Rahmati-Ahmadabad S. The Effect of Aerobic Exercise on SREBP-1c Gene Expression in Skeletal Muscle in Obese Female Rats. Thrita. 2023;12(1). [DOI:10.5812/thrita-138382]
12. Muñoz V, Gaspar R, Mancini M, de Lima R, Vieira R, Crisol B, et al. Short-term physical exercise controls age-related hyperinsulinemia and improves hepatic metabolism in aged rodents. Journal of Endocrinological Investigation. 2023;46(4):815-27. [DOI:10.1007/s40618-022-01947-8]
13. Kazemi Nasab F, Marandi SM, Shirkhani S, Sheikhanian Poor A, Ghaedi K. The Effect of 8 Weeks Aerobic Exercise on LXRa, PEPCK, and G6PC2 mRNA in Obese Prediabetic Mice. Sport Physiology. 2020;12(48):17-38.
14. Mahmood T, Yang P-C. Western blot: technique, theory, and trouble shooting. North American journal of medical sciences. 2012;4(9):429. [DOI:10.4103/1947-2714.100998]
15. Hardie DG, Carling D. The AMP‐activated protein kinase: Fuel gauge of the mammalian cell? European journal of biochemistry. 1997;246(2):259-73. [DOI:10.1111/j.1432-1033.1997.00259.x]
16. Horton JD, Goldstein JL, Brown MS. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. The Journal of clinical investigation. 2002;109(9):1125-31. [DOI:10.1172/JCI0215593]
17. Fischer AH, Jacobson KA, Rose J, Zeller R. Hematoxylin and eosin staining of tissue and cell sections. Cold spring harbor protocols. 2008;2008(5):pdb. prot4986. [DOI:10.1101/pdb.prot4986]
18. Bancroft JD, Gamble M. Theory and practice of histological techniques: Elsevier health sciences; 2008.
19. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 1976;72(1-2):248-54. [DOI:10.1016/0003-2697(76)90527-3]
20. Gripp F, Nava RC, Cassilhas RC, Esteves EA, Magalhães COD, Dias-Peixoto MF, et al. HIIT is superior than MICT on cardiometabolic health during training and detraining. European journal of applied physiology. 2021;121:159-72. [DOI:10.1007/s00421-020-04502-6]
21. Sozen E, Demirel-Yalciner T, Sari D, Avcilar C, Samanci TF, Ozer NK. Deficiency of SREBP1c modulates autophagy mediated lipid droplet catabolism during oleic acid induced steatosis. Metabolism Open. 2021;12:100138. [DOI:10.1016/j.metop.2021.100138]
22. Aoi W, Naito Y, Takanami Y, Ishii T, Kawai Y, Akagiri S, et al. Astaxanthin improves muscle lipid metabolism in exercise via inhibitory effect of oxidative CPT I modification. Biochemical and Biophysical Research Communications. 2008;366(4):892-7. [DOI:10.1016/j.bbrc.2007.12.019]
23. Raza GS, Kaya Y, Stenbäck V, Sharma R, Sodum N, Mutt SJ, et al. Effect of Aerobic Exercise and Time‐Restricted Feeding on Metabolic Markers and Circadian Rhythm in Mice Fed with the High‐Fat Diet. Molecular Nutrition & Food Research. 2024;68(5):2300465. [DOI:10.1002/mnfr.202300465]
24. Amri J, Parastesh M, Sadegh M, Latifi S, Alaee M. High-intensity interval training improved fasting blood glucose and lipid profiles in type 2 diabetic rats more than endurance training; possible involvement of irisin and betatrophin. Physiology international. 2019;106(3):213-24. [DOI:10.1556/2060.106.2019.24]
25. Ma S, Yang J, Tominaga T, Liu C, Suzuki K. A low-carbohydrate ketogenic diet and treadmill training enhanced fatty acid oxidation capacity but did not enhance maximal exercise capacity in mice. Nutrients. 2021;13(2):611. [DOI:10.3390/nu13020611]
26. Deja S, Fletcher JA, Kim C-W, Kucejova B, Fu X, Mizerska M, et al. Hepatic malonyl-CoA synthesis restrains gluconeogenesis by suppressing fat oxidation, pyruvate carboxylation, and amino acid availability. Cell Metabolism. 2024;36(5):1088-104. e12. [DOI:10.1016/j.cmet.2024.02.004]
27. Joseph JS, Ayeleso AO, Mukwevho E. Role of exercise-induced calmodulin protein kinase (CaMK) II activation in the regulation of omega-6 fatty acids and lipid metabolism genes in rat skeletal muscle. Physiol Res. 2017;66(6):969-77. [DOI:10.33549/physiolres.933509]
28. Joseph JS, Fagbohun OF. Exercise increases the expression of glucose transport and lipid metabolism genes at optimum level time point 6 h post-exercise in rat skeletal muscle. Comparative Clinical Pathology. 2022;31(1):147-53. [DOI:10.1007/s00580-022-03318-4]
29. Shahouzehi B, Masoumi-Ardakani Y, Nazari-Robati M, Aminizadeh S. The effect of high-intensity interval training and l-carnitine on the expression of genes involved in lipid and glucose metabolism in the liver of wistar rats. Brazilian Archives of Biology and Technology. 2022;66:e23220100. [DOI:10.1590/1678-4324-2023220100]
30. Townsend LK, Steinberg GR. AMPK and the Endocrine Control of Metabolism. Endocrine reviews. 2023;44(5):910-33. [DOI:10.1210/endrev/bnad012]
Send email to the article author
| Rights and permissions | |
 | This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons Attribution 4.0 International License.
Designed & Developed by : Yektaweb