Showing 1 results for S. Aureus
Pournajaf A, Shokri M, Yaghoubi S, Jafari M, Kamran K, Ghasemi A, Hasanzadeh A, Mirhafez S,
Volume 12, Issue 2 (8-2024)
Abstract
Background & Aim: Mupirocin is used to decolonize Staphylococcus aureus using inhibiting protein synthesis. Therefore, the purpose of this study is to investigate the pattern of antibiotic resistance and determine the phenotypic and genotypic resistance to mupirocin in S. aureus isolated from nasal carriers.
Methods: In this cross-sectional study, 121 nasal swabs were collected from healthcare workers. Methicillin-resistant strains were tested using cefoxitin disk in the antibiogram test and mecA gene amplification in PCR method. E-test method was performed to check high and low level resistance to mupirocin and finally the genes encoding resistance to mupirocin including ileS-1, mupA and mupB were identified using PCR.
Results: 68 isolates (56.2%) of S. aureus were isolated, of which 42 isolates (61.8%) were MRSA. Also, the highest and lowest levels of resistance were related to tetracycline (70.1%) and gentamicin (16.2%). Only, 7 isolates (16.7%) of MRSA and 2 isolates (7.6%) of S. aureus sensitive to methicillin were resistant to mupirocin. MIC E-test results showed that 7 and 2 strains had high and low level resistance to mupirocin, respectively. Also, one strain (11.1%) had iles-1 gene and 5 strains (55.5%) had mupA gene.
Conclusion: The results showed a relatively high prevalence of colonization and antibiotic resistance in S. aureus strains isolated from healthcare workers; therefore, it is essential to carry out further studies and necessary measures to reduce the amount of carriers.
