Volume 11, Issue 2 (9-2023)
2023, 11(2): 56-70 |
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D S, E S, H A. Study on antimicrobial effect of Artemisia Turcomanica ethanolic extract into Klebsiella pneumoniae antibiotic-resistant strains and Its effect on rmpA and fimH drug-resistance genes expression. Journal title 2023; 11 (2) :56-70
URL: http://jms.thums.ac.ir/article-1-1219-en.html
URL: http://jms.thums.ac.ir/article-1-1219-en.html
1- Islamic Azad University, North Tehran Branch
Abstract: (888 Views)
Background & Aim: By antibiotic resistance growing and improvement of resistant bacteria strains, was reported useing of plant extracts considering. The aim of this study was to identification the antibacterial effect of Artemisia turcomanica on Klebsiella pneumoniae resistant strains and its effect of fimH and rmpA drug resistance genes expression.
Methods: From 100 clinical samples were collected, Klebsiella pneumoniae strains were detected by biochemical tests and their antibiotic resistance was identified by disk diffusion test. The presence of fimH and rmpA genes was found using PCR technique. After of Artemisia turcomanica extraction, its antibacterial effect was evaluated of different concentrations against resistance strains. Expression of fimH and rmpA genes with the Real-Time PCR method, before and after of treatment with herbal extract was investigated. Data by SPSS software was analyzed.
Results: Among the 100 isolates, 30 isolates were identified as Klebsiella pneumoniae and from these 30 isolates, 10 isolates with resistance to several antibiotics were selected. The fimH and rmpA genes in eight and seven resistant strains, respectively, were existed. The most resistant strains against the Artemisia turcomanica extract were found with MIC equal to 200 μg/ml. The Real-Time PCR results shown a significant decrease in the fimH and rmpA genes expression after of herbal extract treatment (P<0.001).
Conclusion: According of these results, Turkmen herb could be given antibacterial effect with decrease of resistance genes expression in Klebsiella pneumoniae. For confirming of these results, requests more research.
Methods: From 100 clinical samples were collected, Klebsiella pneumoniae strains were detected by biochemical tests and their antibiotic resistance was identified by disk diffusion test. The presence of fimH and rmpA genes was found using PCR technique. After of Artemisia turcomanica extraction, its antibacterial effect was evaluated of different concentrations against resistance strains. Expression of fimH and rmpA genes with the Real-Time PCR method, before and after of treatment with herbal extract was investigated. Data by SPSS software was analyzed.
Results: Among the 100 isolates, 30 isolates were identified as Klebsiella pneumoniae and from these 30 isolates, 10 isolates with resistance to several antibiotics were selected. The fimH and rmpA genes in eight and seven resistant strains, respectively, were existed. The most resistant strains against the Artemisia turcomanica extract were found with MIC equal to 200 μg/ml. The Real-Time PCR results shown a significant decrease in the fimH and rmpA genes expression after of herbal extract treatment (P<0.001).
Conclusion: According of these results, Turkmen herb could be given antibacterial effect with decrease of resistance genes expression in Klebsiella pneumoniae. For confirming of these results, requests more research.
Keywords: Artemisia turkmanica, Klebsiella pneumoniae, antimicrobial effect, gene expression, antibiotic resistanc
Type of Study: Research |
Subject:
Special
Received: 2023/10/7 | Accepted: 2023/11/22 | Published: 2024/01/31
Received: 2023/10/7 | Accepted: 2023/11/22 | Published: 2024/01/31
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