Volume 12, Issue 1 (6-2024)
مجله دانشگاه علوم پزشکی تربت حیدریه 2024, 12(1): 44-53 |
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A N, B M, M K, R S, M D. Investigating the prevalence of Sarcocystis in slaughterhouses of Esfarayen by digestion method. مجله دانشگاه علوم پزشکی تربت حیدریه 2024; 12 (1) :44-53
URL: http://jms.thums.ac.ir/article-1-1284-en.html
URL: http://jms.thums.ac.ir/article-1-1284-en.html
1- Department of Environmental Health Engineering, Esfarayen Faculty of Medical Science, Esfarayen, Iran
2- Department of Microbiology, Esfarayen Faculty of Medical Science, Esfarayen, Iran
3- Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran & Student Research Committee, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, kerman, Iran
4- Department of Parasitology and Mycology, School of Medical Science, Zabol University of Medical Science, Zabol, Iran
2- Department of Microbiology, Esfarayen Faculty of Medical Science, Esfarayen, Iran
3- Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran & Student Research Committee, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, kerman, Iran
4- Department of Parasitology and Mycology, School of Medical Science, Zabol University of Medical Science, Zabol, Iran
Abstract: (181 Views)
Background & Aim: The prevalence of parasitic protozoan raises severe concerns for food safety and public health, thereby underscoring the crucial importance of understanding its extent. This study investigates Sarcocystis contamination in sheep, goat, and cow carcasses from a slaughterhouse in Esfarayen, northeastern of Iran.
Methods: In this descriptive study, 205 corpses were examined over a year. Sarcocystis bradyzoites were isolated by digestion method. This process involved crushing meat samples obtained from slaughtered animals, digesting them with a 50 ml digestive solution, and incubating them at a precise 40°C for one hour. The filtered solution was centrifuged, and the sediment was stained with Giemsa. Additionally, tissue samples were meticulously viewed for pathological examination and stained with hematoxylin and eosin histopathological sections.
Results: This study showed that out of 205 samples, 136 (66%) were infected by Sarcocystis. The infection was mostly seen in sheep (63.6%). Based on Fischer's accurate test, there was no statistically significant relationship between animal sex and infection rate (p> 0.05). Also, there was a relationship between the goats' weight criteria and the contamination rate (p<0.05).
Conclusion: The results indicate that Esfarayen has a higher Sarcocystis infection rate in traditionally bred slaughtered livestock compared to the rest of the country. There was also a relationship between the research goats' body weight and the degree of contamination.
Methods: In this descriptive study, 205 corpses were examined over a year. Sarcocystis bradyzoites were isolated by digestion method. This process involved crushing meat samples obtained from slaughtered animals, digesting them with a 50 ml digestive solution, and incubating them at a precise 40°C for one hour. The filtered solution was centrifuged, and the sediment was stained with Giemsa. Additionally, tissue samples were meticulously viewed for pathological examination and stained with hematoxylin and eosin histopathological sections.
Results: This study showed that out of 205 samples, 136 (66%) were infected by Sarcocystis. The infection was mostly seen in sheep (63.6%). Based on Fischer's accurate test, there was no statistically significant relationship between animal sex and infection rate (p> 0.05). Also, there was a relationship between the goats' weight criteria and the contamination rate (p<0.05).
Conclusion: The results indicate that Esfarayen has a higher Sarcocystis infection rate in traditionally bred slaughtered livestock compared to the rest of the country. There was also a relationship between the research goats' body weight and the degree of contamination.
Type of Study: Research |
Subject:
Special
Received: 2024/04/5 | Accepted: 2024/05/29 | Published: 2024/07/24
Received: 2024/04/5 | Accepted: 2024/05/29 | Published: 2024/07/24
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