Volume 12, Issue 2 (8-2024)
jmsthums 2024, 12(2): 1-15 |
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SH S, M R, M A K, M H, O A. Investigation of biofilm formation and virulence factors in Pseudomonas aeruginosa isolates from urinary tract infection patients in Tehran hospitals. jmsthums 2024; 12 (2) :1-15
URL: http://jms.thums.ac.ir/article-1-1325-en.html
URL: http://jms.thums.ac.ir/article-1-1325-en.html
1- Pasteur Institute of Iran, Tehran, Iran
2- Pasteur Institute of Iran, Tehran
3- Torbat Heydariyeh University of Medical Sciences
2- Pasteur Institute of Iran, Tehran
3- Torbat Heydariyeh University of Medical Sciences
Abstract: (382 Views)
Background & Aim:Virulence factors play a crucial role in the ability of Pseudomonas aeruginosa to cause urinary tract infections. This study aimed to identify virulence genes in clinical isolates of P. aeruginosa from two hospitals in Tehran, Iran.
Methods: In this study, 200 P. aeruginosa isolates were collected from urinary tract infections in two general hospitals in Tehran, Iran. The isolates were confirmed using standard laboratory tests. Biofilm formation ability was assessed using a phenotypic method. Furthermore, genes encoding selected virulence factors were identified using polymerase chain reaction (PCR).
Results: 53% of the collected isolates exhibited strong biofilm production. The presence of algD, lasB, pcrV, aprA, plcN, toxA, exoS, pilB, plcH and exoU genes was observed in 90%, 88%, 85%, 84%, 80%, 77%, 66%, 62%, 58%, and 45% of the isolates, respectively. A significant association was observed between the presence of the exoS virulence gene and patient age (P=0.04). Furthermore, a significant association was observed between the presence of all investigated genes (except plcN and toxA) and the type of hospital. When investigating the relationship between virulence gene type and biofilm formation intensity, a significant association was observed between the presence of the exoS gene and biofilm formation capacity (p=0.03).
Conclusion: This study demonstrated that P. aeruginosa isolated from urinary tract infections possess virulence genes and a high capacity for biofilm formation. This finding suggests that P. aeruginosa could pose significant challenges in treatment and increase the risk of infection and mortality in patients.
Methods: In this study, 200 P. aeruginosa isolates were collected from urinary tract infections in two general hospitals in Tehran, Iran. The isolates were confirmed using standard laboratory tests. Biofilm formation ability was assessed using a phenotypic method. Furthermore, genes encoding selected virulence factors were identified using polymerase chain reaction (PCR).
Results: 53% of the collected isolates exhibited strong biofilm production. The presence of algD, lasB, pcrV, aprA, plcN, toxA, exoS, pilB, plcH and exoU genes was observed in 90%, 88%, 85%, 84%, 80%, 77%, 66%, 62%, 58%, and 45% of the isolates, respectively. A significant association was observed between the presence of the exoS virulence gene and patient age (P=0.04). Furthermore, a significant association was observed between the presence of all investigated genes (except plcN and toxA) and the type of hospital. When investigating the relationship between virulence gene type and biofilm formation intensity, a significant association was observed between the presence of the exoS gene and biofilm formation capacity (p=0.03).
Conclusion: This study demonstrated that P. aeruginosa isolated from urinary tract infections possess virulence genes and a high capacity for biofilm formation. This finding suggests that P. aeruginosa could pose significant challenges in treatment and increase the risk of infection and mortality in patients.
Type of Study: Research |
Subject:
Special
Received: 2024/07/11 | Accepted: 2024/08/19 | Published: 2024/10/1
Received: 2024/07/11 | Accepted: 2024/08/19 | Published: 2024/10/1
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