Volume 12, Issue 2 (8-2024)                   jmsthums 2024, 12(2): 1-15 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

SH S, M R, M A K, M H, O A. Investigation of biofilm formation and virulence factors in Pseudomonas aeruginosa isolates from urinary tract infection patients in Tehran hospitals. jmsthums 2024; 12 (2) :1-15
URL: http://jms.thums.ac.ir/article-1-1325-en.html
1- Pasteur Institute of Iran, Tehran, Iran
2- Pasteur Institute of Iran, Tehran
3- Torbat Heydariyeh University of Medical Sciences
Abstract:   (492 Views)
Background & Aim:Virulence factors play a crucial role in the ability of Pseudomonas aeruginosa to cause urinary tract infections. This study aimed to identify virulence genes in clinical isolates of P. aeruginosa from two hospitals in Tehran, Iran.
Methods: In this study, 200 P. aeruginosa isolates were collected from urinary tract infections in two general hospitals in Tehran, Iran. The isolates were confirmed using standard laboratory tests. Biofilm formation ability was assessed using a phenotypic method. Furthermore, genes encoding selected virulence factors were identified using polymerase chain reaction (PCR).
Results: 53% of the collected isolates exhibited strong biofilm production. The presence of algD, lasB, pcrV, aprA, plcN, toxA, exoS, pilB, plcH and exoU genes was observed in 90%, 88%, 85%, 84%, 80%, 77%, 66%, 62%, 58%, and 45% of the isolates, respectively. A significant association was observed between the presence of the exoS virulence gene and patient age (P=0.04). Furthermore, a significant association was observed between the presence of all investigated genes (except plcN and toxA) and the type of hospital. When investigating the relationship between virulence gene type and biofilm formation intensity, a significant association was observed between the presence of the exoS gene and biofilm formation capacity (p=0.03).
 Conclusion: This study demonstrated that P. aeruginosa isolated from urinary tract infections possess virulence genes and a high capacity for biofilm formation. This finding suggests that P. aeruginosa could pose significant challenges in treatment and increase the risk of infection and mortality in patients.
 
Full-Text [PDF 443 kb]   (456 Downloads)    
Type of Study: Research | Subject: Special
Received: 2024/07/11 | Accepted: 2024/08/19 | Published: 2024/10/1

References
1. Rahimzadeh M, Shahbazi S, Sabzi S, Habibi M, Asadi Karam MR. Antibiotic resistance and genetic diversity among Pseudomonas aeruginosa isolated from urinary tract infections in Iran. Future Microbiology. 2023;18(16):1171-83. [DOI:10.2217/fmb-2023-0118]
2. Pang Z, Raudonis R, Glick BR, Lin T-J, Cheng Z. Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnology advances. 2019;37(1):177-92. [DOI:10.1016/j.biotechadv.2018.11.013]
3. Edward EA, El Shehawy MR, Abouelfetouh A, Aboulmagd E. Prevalence of different virulence factors and their association with antimicrobial resistance among Pseudomonas aeruginosa clinical isolates from Egypt. BMC microbiology. 2023;23(1):161. [DOI:10.1186/s12866-023-02897-8]
4. Parvaei M, Habibi M, Shahbazi S, Babaluei M, Farokhi M, Karam MRA. Immunostimulatory chimeric protein encapsulated in gelatin nanoparticles elicits protective immunity against Pseudomonas aeruginosa respiratory tract infection. International Journal of Biological Macromolecules. 2024:133964. [DOI:10.1016/j.ijbiomac.2024.133964]
5. Liao C, Huang X, Wang Q, Yao D, Lu W. Virulence factors of Pseudomonas aeruginosa and antivirulence strategies to combat its drug resistance. Frontiers in cellular and infection microbiology. 2022;12:926758. [DOI:10.3389/fcimb.2022.926758]
6. Heydari S, Eftekhar F. Biofilm formation and β-lactamase production in burn isolates of Pseudomonas aeruginosa. Jundishapur journal of microbiology. 2015;8(3). [DOI:10.5812/jjm.15514]
7. Habibi A, Honarmand R. Profile of virulence factors in the multi-drug resistant Pseudomonas aeruginosa strains of human urinary tract infections (UTI). Iranian Red Crescent Medical Journal. 2015;17(12). [DOI:10.5812/ircmj.26095]
8. Ullah W, Qasim M, Rahman H, Jie Y, Muhammad N. Beta-lactamase-producing Pseudomonas aeruginosa: Phenotypic characteristics and molecular identification of virulence genes. Journal of the Chinese Medical Association. 2017;80(3):173-7. [DOI:10.1016/j.jcma.2016.08.011]
9. Cotar A-I, Chifiriuc M-C, Dinu S, Bucur M, Iordache C, Banu O, et al. Screening of molecular virulence markers in Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from clinical infections. International journal of molecular sciences. 2010;11(12):5273-91. [DOI:10.3390/ijms11125273]
10. Sabharwal N, Dhall S, Chhibber S, Harjai K. Molecular detection of virulence genes as markers in Pseudomonas aeruginosa isolated from urinary tract infections. International journal of molecular epidemiology and genetics. 2014;5(3):125.
11. Lynch SV, Flanagan JL, Sawa T, Fang A, Baek MS, Rubio-Mills A, et al. Polymorphisms in the Pseudomonas aeruginosa type III secretion protein, PcrV-implications for anti-PcrV immunotherapy. Microbial Pathogenesis. 2010;48(6):197-204. [DOI:10.1016/j.micpath.2010.02.008]
12. Olejnízková K, Hola V. The comparison of selected virulence factors in Pseudomonas aeruginosa catheter isolates. Epidemiologie, Mikrobiologie, Imunologie: Casopis Spolecnosti pro Epidemiologii a Mikrobiologii Ceske Lekarske Spolecnosti JE Purkyne. 2012;61(1-2):21-8.
13. Lima JLdC, Alves LR, Jacomé PRLdA, Bezerra JP, Maciel MAV, Morais MMCd. Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing. Brazilian Journal of Infectious Diseases. 2018;22(2):129-36. [DOI:10.1016/j.bjid.2018.03.003]
14. da Silva Carvalho T, Perez LRR. Impact of biofilm production on polymyxin B susceptibility among Pseudomonas aeruginosa clinical isolates. Infection Control & Hospital Epidemiology. 2019;40(6):739-40. [DOI:10.1017/ice.2019.85]
15. Dorri K, Modarresi F, Shakibaie M, Moazamian E. Frequency of Gene-Producing Strains (aprA, rhlI, rhlR, algD) in Clinical Isolates of Pseudomonas aeruginosa, Isolated from Hospitals of South Fars. Pars Journal of Medical Sciences. 2022;20(2):39-47.
16. Bazghandi SA, Arzanlou M, Peeridogaheh H, Vaez H, Sahebkar A, Khademi F. Prevalence of virulence genes and drug resistance profiles of Pseudomonas aeruginosa isolated from clinical specimens. Jundishapur Journal of Microbiology. 2021;14(8). [DOI:10.5812/jjm.118452]
17. Faraji F, Mahzounieh M, Ebrahimi A, Fallah F, Teymournejad O, Lajevardi B. Molecular detection of virulence genes in Pseudomonas aeruginosa isolated from children with Cystic Fibrosis and burn wounds in Iran. Microbial pathogenesis. 2016;99:1-4. [DOI:10.1016/j.micpath.2016.07.013]
18. Heidary Z, Bandani E, Eftekhary M, Jafari AA. Virulence genes profile of multidrug resistant Pseudomonas aeruginosa isolated from Iranian children with UTIs. Acta Medica Iranica. 2016:201-10.
19. Amirmozafari N, Mehrabadi JF, Habibi A. Association of the exotoxin A and exoenzyme S with antimicrobial resistance in Pseudomonas aeruginosa strains. Archives of Iranian Medicine. 2016;19(5):0-.
20. Badamchi A, Masoumi H, Javadinia S, Asgarian R, Tabatabaee A. Molecular detection of six virulence genes in Pseudomonas aeruginosa isolates detected in children with urinary tract infection. Microbial pathogenesis. 2017;107:44-7. [DOI:10.1016/j.micpath.2017.03.009]
21. Mohammadzadeh A, Mardaneh J, Ahmadi R, Adabi J. Evaluation of the virulence features and antibiotic resistance patterns of pathogenic Pseudomonas aeruginosa strains isolated from hospitalized patients in Gonabad, Iran. Archives of Pediatric Infectious Diseases. 2017;5(3). [DOI:10.5812/pedinfect.41267]
22. Nikbin V, Aslani MM, Sharafi Z, Hashemipour M, Shahcheraghi F, Ebrahimipour G. Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins. Iranian journal of microbiology. 2012;4(3):118.
23. Pournajaf A, Razavi S, Irajian G, Ardebili A, Erfani Y, Solgi S, et al. Integron types, antimicrobial resistance genes, virulence gene profile, alginate production and biofilm formation in Iranian cystic fibrosis Pseudomonas aeruginosa isolates. Infez Med. 2018;26(3):226-36.
24. Kiyaga S, Kyany'a C, Muraya AW, Smith HJ, Mills EG, Kibet C, et al. Genetic diversity, distribution, and genomic characterization of antibiotic resistance and virulence of clinical Pseudomonas aeruginosa Strains in Kenya. Frontiers in Microbiology. 2022;13:835403. [DOI:10.3389/fmicb.2022.835403]
25. Peña C, Cabot G, Gómez-Zorrilla S, Zamorano L, Ocampo-Sosa A, Murillas J, et al. Influence of virulence genotype and resistance profile in the mortality of Pseudomonas aeruginosa bloodstream infections. Clinical Infectious Diseases. 2015;60(4):539-48. [DOI:10.1093/cid/ciu866]
26. Horna G, Quezada K, Ramos S, Mosqueda N, Rubio M, Guerra H, et al. Specific type IV pili groups in clinical isolates of Pseudomonas aeruginosa. International Microbiology. 2019;22:131-41. [DOI:10.1007/s10123-018-00035-3]
27. Bahador N, Shoja S, Faridi F, Dozandeh-Mobarrez B, Qeshmi FI, Javadpour S, et al. Molecular detection of virulence factors and biofilm formation in Pseudomonas aeruginosa obtained from different clinical specimens in Bandar Abbas. Iranian Journal of Microbiology. 2019;11(1):25. [DOI:10.18502/ijm.v11i1.701]
28. Magalhães MJTL, Pontes G, Serra PT, Balieiro A, Castro D, Pieri FA, et al. Multidrug resistant Pseudomonas aeruginosa survey in a stream receiving effluents from ineffective wastewater hospital plants. BMC microbiology. 2016;16:1-8. [DOI:10.1186/s12866-016-0798-0]
29. Corehtash ZG, Khorshidi A, Firoozeh F, Akbari H, Aznaveh AM. Biofilm formation and virulence factors among Pseudomonas aeruginosa isolated from burn patients. Jundishapur journal of microbiology. 2015;8(10). [DOI:10.5812/jjm.22345]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Designed & Developed by : Yektaweb