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jmsthums 2025, 13(1): 33-45 |
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M H S, M M, M A, H F, R R, D I et al . Comparison of the effects of hydro-alcoholic extracts of marigold, Aloe vera, and castor on the proliferation of L929 cells. jmsthums 2025; 13 (1) :33-45
URL: http://jms.thums.ac.ir/article-1-1318-en.html
URL: http://jms.thums.ac.ir/article-1-1318-en.html
Hosseini Soumaiegi M1 
, Masoudi M2 , Ahmadyar M3 , Fazelinejad H4 , Rahbarian R5 , Ilbeigi D6 , Fahmidehkar M 6
, Masoudi M2 , Ahmadyar M3 , Fazelinejad H4 , Rahbarian R5 , Ilbeigi D6 , Fahmidehkar M 6
1- Department of Biology, Payame Noor University, Mashhad, Iran
2- Vice Chancellery of Education and Research, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
3- Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4- Department of Biology, Hakim Sabzevari University, Sabzevar, Iran & Research Core of Cognitive Sciences and Aging Studies, Hakim Sabzevari University, Sabzevar, Iran
5- Department of Biology, Payame Noor University, Tehran, Iran
6- Department of Clinical Biochemistry, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
2- Vice Chancellery of Education and Research, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
3- Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4- Department of Biology, Hakim Sabzevari University, Sabzevar, Iran & Research Core of Cognitive Sciences and Aging Studies, Hakim Sabzevari University, Sabzevar, Iran
5- Department of Biology, Payame Noor University, Tehran, Iran
6- Department of Clinical Biochemistry, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Abstract: (199 Views)
Background & Aim: The skin plays a crucial role in protecting against water loss, bleeding, and fighting microorganisms. The use of medicinal plants has gained global attention due to their minimal side effects, cost-effectiveness, and significant therapeutic benefits. The World Health Organization has also emphasized the importance of developing industries related to the cultivation of medicinal plants. This study aims to evaluate the effects of hydro-alcoholic extracts of Aloe Vera, Castor, and Calendula on the proliferation and growth of L929 cells.
Methods: L929 cells were cultured in RPMI 1640 medium and incubated with various concentrations of hydro-alcoholic extracts. The IC50 for each extract was determined using the MTT assay. Subsequently, cell proliferation was evaluated using the MTT assay and the Wound Healing assay at concentrations significantly lower than the IC50.
Results: The MTT assay showed the highest proliferative effect at a concentration of 1 mg/ml. After 48 hours of treatment, the number of L929 cells increased significantly in the groups treated with Aloe vera, Ricinus communis, and Calendula officinalis, with reported values of 0.61±0.006, 0.57±0.007, and 0.55±0.006, respectively. These values were significantly higher than the control group (0.45±0.006) (P<0.05). In terms of cell migration and wound healing, the average scratch width 48 hours after treatment with Aloe vera and Ricinus communis was 50.54±3.26 and 57.82±4.12, respectively, which showed a significant reduction compared to the control group (68.92±2.73) (P<0.05).
Conclusion: Based on our findings, it can be concluded that Aloe vera has a significantly greater and more effective impact on the growth of L929 cells compared to the other extracts studied. This suggests that incorporating Aloe vera into pharmaceutical formulations for wound healing may have potential benefits.
Methods: L929 cells were cultured in RPMI 1640 medium and incubated with various concentrations of hydro-alcoholic extracts. The IC50 for each extract was determined using the MTT assay. Subsequently, cell proliferation was evaluated using the MTT assay and the Wound Healing assay at concentrations significantly lower than the IC50.
Results: The MTT assay showed the highest proliferative effect at a concentration of 1 mg/ml. After 48 hours of treatment, the number of L929 cells increased significantly in the groups treated with Aloe vera, Ricinus communis, and Calendula officinalis, with reported values of 0.61±0.006, 0.57±0.007, and 0.55±0.006, respectively. These values were significantly higher than the control group (0.45±0.006) (P<0.05). In terms of cell migration and wound healing, the average scratch width 48 hours after treatment with Aloe vera and Ricinus communis was 50.54±3.26 and 57.82±4.12, respectively, which showed a significant reduction compared to the control group (68.92±2.73) (P<0.05).
Conclusion: Based on our findings, it can be concluded that Aloe vera has a significantly greater and more effective impact on the growth of L929 cells compared to the other extracts studied. This suggests that incorporating Aloe vera into pharmaceutical formulations for wound healing may have potential benefits.
Type of Study: Research |
Subject:
Special
Received: 2024/07/25 | Accepted: 2025/03/3 | Published: 2025/06/30
Received: 2024/07/25 | Accepted: 2025/03/3 | Published: 2025/06/30
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