Volume 13, Issue 1 (6-2025)                   jmsthums 2025, 13(1): 33-45 | Back to browse issues page

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M H S, M M, M A, H F, R R, D I et al . Comparison of the effects of hydro-alcoholic extracts of marigold, Aloe vera, and castor on the proliferation of L929 cells. jmsthums 2025; 13 (1) :33-45
URL: http://jms.thums.ac.ir/article-1-1318-en.html
1- Department of Biology, Payame Noor University, Mashhad, Iran
2- Vice Chancellery of Education and Research, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
3- Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4- Department of Biology, Hakim Sabzevari University, Sabzevar, Iran & Research Core of Cognitive Sciences and Aging Studies, Hakim Sabzevari University, Sabzevar, Iran
5- Department of Biology, Payame Noor University, Tehran, Iran
6- Department of Clinical Biochemistry, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Abstract:   (39 Views)
Background & Aim: The skin plays a crucial role in protecting against water loss, bleeding, and fighting microorganisms. The use of medicinal plants has gained global attention due to their minimal side effects, cost-effectiveness, and significant therapeutic benefits. The World Health Organization has also emphasized the importance of developing industries related to the cultivation of medicinal plants. This study aims to evaluate the effects of hydro-alcoholic extracts of Aloe Vera, Castor, and Calendula on the proliferation and growth of L929 cells.
Methods: L929 cells were cultured in RPMI 1640 medium and incubated with various concentrations of hydro-alcoholic extracts. The IC50 for each extract was determined using the MTT assay. Subsequently, cell proliferation was evaluated using the MTT assay and the Wound Healing assay at concentrations significantly lower than the IC50.
Results: The MTT assay showed the highest proliferative effect at a concentration of 1 mg/ml. After 48 hours of treatment, the number of L929 cells increased significantly in the groups treated with Aloe vera, Ricinus communis, and Calendula officinalis, with reported values of 0.61±0.006, 0.57±0.007, and 0.55±0.006, respectively. These values were significantly higher than the control group (0.45±0.006) (P<0.05). In terms of cell migration and wound healing, the average scratch width 48 hours after treatment with Aloe vera and Ricinus communis was 50.54±3.26 and 57.82±4.12, respectively, which showed a significant reduction compared to the control group (68.92±2.73) (P<0.05).
Conclusion: Based on our findings, it can be concluded that Aloe vera has a significantly greater and more effective impact on the growth of L929 cells compared to the other extracts studied. This suggests that incorporating Aloe vera into pharmaceutical formulations for wound healing may have potential benefits.
Full-Text [PDF 445 kb]   (11 Downloads)    
Type of Study: Research | Subject: Special
Received: 2024/07/25 | Accepted: 2025/03/3 | Published: 2025/06/30

References
1. Bolla SR, Al-Subaie AM, Al-Jindan RY, Balakrishna JP, Ravi PK, Veeraraghavan VP, et al. (2019) In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression. Heliyon 5(5):e01648. [DOI:10.1016/j.heliyon.2019.e01648]
2. GHaderi R AM (2014) Recent advances in wound healing management. Journal scientific Birjand University of medical sciences. 21(1):1-19.
3. Bainbridge P (2013) Wound healing and the role of fibroblasts. Journal of wound care 22(8). [DOI:10.12968/jowc.2013.22.8.407]
4. Martin P (1997) Wound healing--aiming for perfect skin regeneration. Science 276(5309):75-81. [DOI:10.1126/science.276.5309.75]
5. George Broughton I, Janis JE, Attinger CE (2006) Wound healing: an overview. Plastic and reconstructive surgery 117(7S):1e-S-32e-S. [DOI:10.1097/01.prs.0000222562.60260.f9]
6. Evans RA, Tian YC, Steadman R, Phillips AO (2003) TGF-β1-mediated fibroblast-myofibroblast terminal differentiation-the role of smad proteins. Experimental cell research 282(2):90-100. [DOI:10.1016/S0014-4827(02)00015-0]
7. Helary C, Ovtracht L, Coulomb B, Godeau G, Giraud-Guille MM (2006) Dense fibrillar collagen matrices: a model to study myofibroblast behaviour during wound healing. Biomaterials 27(25):4443-52. [DOI:10.1016/j.biomaterials.2006.04.005]
8. Prendecka M, Mlak R, Jaszek M, Osinska-Jaroszuk M, Jakubiak-Hulicz M, Leibold C, et al. (2016) Effect of exopolysaccharide from Ganoderma applanatum on the electrical properties of mouse fibroblast cells line L929 culture using an electric cell-substrate impedance sensing (ECIS)-Preliminary study. Annals of Agricultural and Environmental Medicine 23(2). [DOI:10.5604/12321966.1203891]
9. Stojkovic R, Ivankovic S, Ivankovic D, Attias L, Mantovani A, Fucic A (2015) Testosterone-induced micronuclei and increased nuclear division rate in L929 cell line expressing the androgen receptor. Toxicology in Vitro 29(5):1021-5. [DOI:10.1016/j.tiv.2015.04.007]
10. Asghari B, aminian, Ruqiya (2017) Investiga the metabolic content of Different marigold extracts. the first international conference on new technologiesin science
11. Givol O, Kornhaber R, Visentin D, Cleary M, Haik J, Harats M (2019) A systematic review of Calendula officinalis extract for wound healing. Wound Repair and Regeneration 27(5):548-61. [DOI:10.1111/wrr.12737]
12. Nitbani FO, Tjitda PJP, Wogo HE, Detha AIR (2022) Preparation of Ricinoleic Acid from Castor Oil: A Review. Journal of Oleo Science 71(6):781-93. [DOI:10.5650/jos.ess21226]
13. Hosseini k, Khalil (2010) Investiga ting the chemical and medicinal compounds in the yellow patience plant Aloe vera.
14. Joseph B, Raj SJ (2010) Pharmacognostic and phytochemical properties of Aloe vera linn an overview. Int J Pharm Sci Rev Res 4(2):106-10.
15. Najafi N, Arabi M, Jafarzadeh H (2014) Inducing effect of aloe vera gel extract on epithelial growth factor receptor gene expression in cutaneous wounds of male mice Balb/c.
16. Spector DL, Goldman RD, Leinwand LA. Cells: a laboratory manual: Cold Spring Harbor Laboratory Press; 1998.
17. Zare M, Ebrahimi Vosta Kalaee S, Aezi H (2020) Analysis the Cytotoxicity Effects of Hydroalcoholic Extract of Danae Racemosa Plant on Mouse Breast Cancer Cells (4T1) by MTT. Knowledge and Health 15(1):49-57.
18. Najafi N, Arabi M, Jafarzadeh H (2014) The healing power of Aloe vera mucilage: induction of insulin-like growth factor gene expression and regeneration tissue in mouse damaged skin. Journal of Shahrekord University of Medical Sciences 16(2).
19. Alahtavakoli M, Vazirinejad R, Ansari Jaberi A, Negahban T, Mashayekhi H, Nazari M, et al. (2012) Effect of Teucrium polium extract on skin wound healing in rat. Hormozgan Medical Journal 16(1):17-24.
20. Manoj K, Mishra D, Maity TK, Gupta SD (2009) Screening wound-healing potential of different Aloe vera L. germplasms at the cellular level. Med Aromat Plant Sci Biotechnol 3(1):62-4.
21. malekhosseini A, Ghaffarzadegan R, Alizadeh SA, GR, Reza, Haji Agaei R, Ahmadlou M (2013) Effect of aloe vera gel, compared to 1% silver sulfadiazine cream on second-degree burn wound healing. Complementary Medicine Journal 3(1):418-28.
22. Choi S, Chung M-H (2003) A review on the relationship between aloe vera components and their biologic effects. Seminars in Integrative Medicine 1(1):53-62. [DOI:10.1016/S1543-1150(03)00005-X]
23. Nada AA, Hassabo AG, Fayad W, Awad HM, Sleem AA, Shaffie NM, et al. (2015) Biomaterials based on essential fatty acids and carbohydrates for chronic wounds. Journal of Applied Pharmaceutical Science 5(3):013-21. [DOI:10.7324/JAPS.2015.510.S3]
24. Davari S, Hajinezhad M, Samadi K, Eftekhari S (2018) The effect of calendula, aloe and caster on cutaneous wounds healing process in mature male rat. Journal of Rafsanjan University of Medical Sciences 17(2):93-104.
25. Lawrence R, Tripathi P, Jeyakumar E (2009) Isolation, purification and evaluation of antibacterial agents from Aloe vera. Brazilian Journal of Microbiology 40:906-15. [DOI:10.1590/S1517-83822009000400023]

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