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jmsthums 2025, 13(1): 33-45 |
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Hosseini Soumaiegi M, Masoudi M, Ahmadyar M E, Fazelinejad H, Rahbarian R, Ilbeigi D et al . Comparative study of hydro-alcoholic extract effects from Marigold, Aloe vera, and Castor on L929 cell proliferation. jmsthums 2025; 13 (1) :33-45
URL: http://jms.thums.ac.ir/article-1-1318-en.html
URL: http://jms.thums.ac.ir/article-1-1318-en.html
Malihe Hosseini Soumaiegi1 
, Maha Masoudi2 , Mohammad Esmail Ahmadyar3 , Hassan Fazelinejad4 , Raheleh Rahbarian5 , Davod Ilbeigi6 , Mohammad Ali Fahmidehkar6
, Maha Masoudi2 , Mohammad Esmail Ahmadyar3 , Hassan Fazelinejad4 , Raheleh Rahbarian5 , Davod Ilbeigi6 , Mohammad Ali Fahmidehkar6
1- Department of Biology, Payame Noor University, Mashhad, Iran
2- Vice Chancellery of Education and Research, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
3- Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4- Department of Biology, Hakim Sabzevari University, Sabzevar, Iran & Research Core of Cognitive Sciences and Aging Studies, Hakim Sabzevari University, Sabzevar, Iran
5- Department of Biology, Payame Noor University, Tehran, Iran
6- Department of Clinical Biochemistry, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
2- Vice Chancellery of Education and Research, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
3- Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4- Department of Biology, Hakim Sabzevari University, Sabzevar, Iran & Research Core of Cognitive Sciences and Aging Studies, Hakim Sabzevari University, Sabzevar, Iran
5- Department of Biology, Payame Noor University, Tehran, Iran
6- Department of Clinical Biochemistry, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Abstract: (790 Views)
Background & Aim: The skin is important for protection against water loss, hemorrhage, and microbial invasion. Growing interest in medicinal plants in the world is due to their reduced side effects, lower financial cost, outstanding therapeutic benefits, and the focus of the World Health Organization on the development of medicinal plant cultivation industries. This study was aimed to compare the effects of hydro-alcoholic extract of Aloe vera, Castor, and Marigold on the proliferation and growth of L929 cells.
Methods: L929 cells were grown in the standard medium of RPMI 1640, followed by incubation of varying concentrations of different hydro-alcoholic extracts. Initially, IC50 values for each extract were determined using the MTT assay. Cell proliferation was then assessed at concentrations much lower than the IC50 in the form of both MTT and wound healing assays. Data analysis was performed using SPSS software version 26 and the level of significance was set at 5%.
Results: Maximum cell proliferation was obtained using the MTT assay at concentrations of the extract of 1 mg/ml. The mean cell counts and proliferation rates at 48 hours after treatment with Aloe vera, Castor, and Marigold were 0.61±0.006, 0.57±0.007, and 0.55±0.006, respectively, with statistically significant increases compared to the control group (0.45±0.006) (P<0.05). Regarding the migration and wound healing outcomes, the average wound width at 48 hours after Aloe vera and Castor treatment was 50.54 ± 3.26 and 57.82 ± 4.12, respectively, which were significantly less than that of the control group (68.92 ± 2.73) (P < 0.05).
Conclusion: Since Aloe vera showed better effects on L929 cell growth than other tested extracts, it would be advantageous to incorporate Aloe vera into the pharmaceutical formulations for wound healing treatment purposes.
Methods: L929 cells were grown in the standard medium of RPMI 1640, followed by incubation of varying concentrations of different hydro-alcoholic extracts. Initially, IC50 values for each extract were determined using the MTT assay. Cell proliferation was then assessed at concentrations much lower than the IC50 in the form of both MTT and wound healing assays. Data analysis was performed using SPSS software version 26 and the level of significance was set at 5%.
Results: Maximum cell proliferation was obtained using the MTT assay at concentrations of the extract of 1 mg/ml. The mean cell counts and proliferation rates at 48 hours after treatment with Aloe vera, Castor, and Marigold were 0.61±0.006, 0.57±0.007, and 0.55±0.006, respectively, with statistically significant increases compared to the control group (0.45±0.006) (P<0.05). Regarding the migration and wound healing outcomes, the average wound width at 48 hours after Aloe vera and Castor treatment was 50.54 ± 3.26 and 57.82 ± 4.12, respectively, which were significantly less than that of the control group (68.92 ± 2.73) (P < 0.05).
Conclusion: Since Aloe vera showed better effects on L929 cell growth than other tested extracts, it would be advantageous to incorporate Aloe vera into the pharmaceutical formulations for wound healing treatment purposes.
Type of Study: Research |
Subject:
Special
Received: 2024/07/25 | Accepted: 2025/02/1 | Published: 2025/03/21
Received: 2024/07/25 | Accepted: 2025/02/1 | Published: 2025/03/21
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